E01A0047 Human Adrenomedullin ELISA kit
Human Adrenomedullin ELISA kit is suitable for the detection of samples from human species. Adrenomedullin can also be called AM, ADM, ADM precursor, ADML.
Specifications of Human Adrenomedullin ELISA kit
Porduct Information | |
Cat. No. | E01A0047 |
Product Name | Human Adrenomedullin ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 100-2500pg/mL |
Sensitivity | 1.0 pg/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 100 pg/mL | 1 vial |
STANDARD C (0.5mL) | 250 pg/mL | 1 vial |
STANDARD D (0.5mL) | 500 pg/mL | 1 vial |
STANDARD E (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD F (0.5mL) | 2500 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
AM ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-AM antibody and an AM-HRP conjugate. The assay sample and buffer are incubated together with AM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AM concentration since AM from samples and AM-HRP conjugate compete for the anti-AM antibody binding site. Since the number of sites is limited, as more sites are occupied by AM from the sample, fewer sites are left to bind AM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AM concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Adrenomedullin ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 89-107% | |
Linearity | Diluent Ratio | Range % |
1:2 | 95-102 | |
1:4 | 90-108 | |
1:8 | 91-112 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between AM and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Human Adrenomedullin ELISA kit
Citations of Human Adrenomedullin ELISA kit
E01A0047 has been referenced in the below publications:
Analysis of the Secretome of Apoptotic Peripheral Blood Mononuclear Cells: Impact of Released Proteins and Exosomes for Tissue Regeneration.
