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BlueGene Biotech Bovine Adenosine Monophosphate Activated Protein ELISA kit_copy19

  • Signal Transduction_copy16_copy20230413_copy20230413_copy20230413

E11A0043 Bovine Adenosine Monophosphate Activated Protein ELISA kit

Bovine Adenosine Monophosphate Activated Protein ELISA kit is suitable for the detection of samples from Bovine species. Adenosine Monophosphate Activated Protein can also be called PRKAa1, AMPKa1, PKA, ACACA kinase, AMPK.

Specifications of Bovine Adenosine Monophosphate Activated Protein ELISA kit_copy16_copy20230413_copy20230413_copy20230413

Product Information

Cat. No.

E11A0043

Product Name

Bovine Adenosine Monophosphate Activated Protein ELISA kit

Species

Bovine

Product Size

48 Tests / 96 Tests

Concentration

250-5000 pg/mL

Sensitivity

1.0 pg/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

250 pg/mL

1 vial

STANDARD C (0.5mL)

500 pg/mL

1 vial

STANDARD D (0.5mL)

1000 pg/mL

1 vial

STANDARD E (0.5mL)

2500 pg/mL

1 vial

STANDARD F (0.5mL)

5000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

AMPK ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-AMPK antibody and an AMPK-HRP conjugate. The assay sample and buffer are incubated together with AMPK-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AMPK concentration since AMPK from samples and AMPK-HRP conjugate compete for the anti-AMPK antibody binding site. Since the number of sites is limited, as more sites are occupied by AMPK from the sample, fewer sites are left to bind AMPK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AMPK concentration in each sample is interpolated from this standard curve.


Quality Control on Bovine Adenosine Monophosphate Activated Protein ELISA kit_copy16_copy20230413_copy20230413_copy20230413

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

87-101%

Linearity

Diluent Ratio

Range %

1:2

85-103

1:4

84-108

1:8

82-107

Specificity/Cross-reactivity

No significant cross-reactivity or interference between AMPK and analogues was observed.


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Summary of the Assay Procedure for Bovine Adenosine Monophosphate Activated Protein ELISA kit_copy16_copy20230413_copy20230413_copy20230413

Summary of the Assay Procedure for Bovine Adenosine Monophosphate Activated Protein ELISA kit_copy16_copy20230413_copy20230413_copy20230413

Citations of Bovine Adenosine Monophosphate Activated Protein ELISA kit_copy16_copy20230413_copy20230413_copy20230413

E11A0043 has been referenced in the below publications:

β-Hydroxybutyric Sodium Salt Inhibition of Growth Hormone and Prolactin Secretion via the cAMP/PKA/CREB and AMPK Signaling Pathways in Dairy Cow Anterior Pituitary Cells.

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