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Bovine Interleukin 2 ELISA kit_copy53

  • Immunology_copy53

E11I0308 Bovine Interleukin 2 ELISA kit

The Bovine Interleukin 2 ELISA kit can be used to identify samples from the bovine species. Interleukin 2 can also be called Interleukin-2, IL-2, T-cell growth factor, Aldesleukin, Lymphokine, POIL2, T Cell Growth Factor, TCGF, IL 2, IL2.

Specifications of Bovine Interleukin 2 ELISA kit_copy51_copy20230414_copy20230414

Product Information

Cat. No.

E11I0308

Product Name

Bovine Interleukin 2 ELISA kit

Species

Bovine

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

IL2 ELISA kit uses an anti-IL2 antibody and an IL2-HRP conjugate in a competitive enzyme immunoassay method. IL2-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IL2 antibody binding site between IL2 from samples and IL2-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IL2. Since the number of sites is limited, as more sites are occupied by IL2 from the sample, fewer sites are left to bind IL2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL2 concentration in each sample is interpolated from this standard curve.


Quality Control on Bovine Interleukin 2 ELISA kit_copy51_copy20230414_copy20230414

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between IL2 and analogues was observed.


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Summary of the Assay Procedure for Bovine Interleukin 2 ELISA kit_copy51_copy20230414_copy20230414

Summary of the Assay Procedure for Bovine Interleukin 2 ELISA kit_copy51_copy20230414_copy20230414

Citations of Bovine Interleukin 2 ELISA kit_copy51_copy20230414_copy20230414

E11I0308 has been referenced in the below publications:

Infection of Cattle With Cryptosporidium parvum: Mast Cell Accumulation in Small Intestine Mucosa.

To separation of lactic acid bacteria and yeast cultures for the application of cow and newborn calf perinatal care.

Cryptosporidium parvum. Protective Immunity against Cryptosporidiosis with Recombinant Lactobacillus Live Vaccines Expressing cp966 after Oral Immunization.

Effects of complex anionic salt premix on production performance and health stadus of perinatal cows.

蒲公英水提物对断奶仔猪免疫功能和血清抗氧化功能的效应试验。

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