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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Bovine Secretory Immunoglobulin A ELISA kit_copy69

  • Immunology_copy66_copy20230414_copy20230414_copy20230414

E11S0003 Bovine Secretory Immunoglobulin A ELISA kit

The Bovine Secretory Immunoglobulin A ELISA kit can be used to identify samples from the bovine species. Secretory Immunoglobulin A can also be called SIgA, secretory IgA.

Specifications of Bovine Secretory Immunoglobulin A ELISA kit_copy66_copy20230414_copy20230414_copy20230414

Product Information

Cat. No.

E11S0003

Product Name

Bovine Secretory Immunoglobulin A ELISA kit

Species

Bovine

Product Size

48 Tests / 96 Tests

Concentration

2.5-50 ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

SIgA ELISA kit uses an anti-SIgA antibody and an SIgA-HRP conjugate in a competitive enzyme immunoassay method. SIgA-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-SIgA antibody binding site between SIgA from samples and SIgA-HRP conjugate, the intensity of the color is inversely proportional to the concentration of SIgA. Since the number of sites is limited, as more sites are occupied by SIgA from the sample, fewer sites are left to bind SIgA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SIgA concentration in each sample is interpolated from this standard curve.


Quality Control on Bovine Secretory Immunoglobulin A ELISA kit_copy66_copy20230414_copy20230414_copy20230414

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between SIgA and analogues was observed.


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Summary of the Assay Procedure for Bovine Secretory Immunoglobulin A ELISA kit_copy66_copy20230414_copy20230414_copy20230414

Summary of the Assay Procedure for Bovine Secretory Immunoglobulin A ELISA kit_copy66_copy20230414_copy20230414_copy20230414

Citations of Bovine Secretory Immunoglobulin A ELISA kit_copy66_copy20230414_copy20230414_copy20230414

E11S0003 has been referenced in the below publications:

Cryptosporidium parvum. Protective Immunity against Cryptosporidiosis with Recombinant Lactobacillus Live Vaccines Expressing cp966 after Oral Immunization.

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