Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Bovine Serum Albumin ELISA kit_copy34

  • Cardiovascular10_copy31_copy20230412_copy20230412_copy20230412

E11S0014 Bovine Serum Albumin ELISA kit

The Bovine Serum Albumin ELISA kit can be used to identify samples from the bovine species. Serum Albumin can also be called SALB, ALB, Serum albumin.

Specifications of Bovine Serum Albumin ELISA kit_copy31_copy20230412_copy20230412_copy20230412

Product Information

Cat. No.

E11S0014

Product Name

Bovine Serum Albumin ELISA kit

Species

Bovine

Product Size

48 Tests / 96 Tests

Concentration

10-250 ug/mL

Sensitivity

1.0 ug/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ug/mL

1 vial

STANDARD B (0.5mL)

10 ug/mL

1 vial

STANDARD C (0.5mL)

25 ug/mL

1 vial

STANDARD D (0.5mL)

50 ug/mL

1 vial

STANDARD E (0.5mL)

100 ug/mL

1 vial

STANDARD F (0.5mL)

250 ug/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

SALB ELISA kit uses an anti-SALB antibody and an SALB-HRP conjugate in a competitive enzyme immunoassay method. SALB-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-SALB antibody binding site between SALB from samples and SALB-HRP conjugate, the intensity of the color is inversely proportional to the concentration of SALB. Since the number of sites is limited, as more sites are occupied by SALB from the sample, fewer sites are left to bind SALB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SALB concentration in each sample is interpolated from this standard curve.


Quality Control on Bovine Serum Albumin ELISA kit_copy31_copy20230412_copy20230412_copy20230412

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between SALB and analogues was observed.


BlueGene Biotech Product Show10_copy31_copy20230412_copy20230412_copy20230412

Summary of the Assay Procedure for Bovine Serum Albumin ELISA kit_copy31_copy20230412_copy20230412_copy20230412

Summary of the Assay Procedure for Bovine Serum Albumin ELISA kit_copy31_copy20230412_copy20230412_copy20230412

Citations of Bovine Serum Albumin ELISA kit_copy31_copy20230412_copy20230412_copy20230412

E11S0014 has been referenced in the below publications:

Study on change of structure characteristics of membrane in ultrafiltration process of protein.

Preparation of a novel sulfonated polyphenlene sulfone with flexible side chain for ultrafiltration membrane application.

Preparation of poly(vinylidene fluoride) (PVDF)/acetalyzed poly(vinyl alcohol) ultrafiltration membrane with the enhanced hydrophilicity and the anti-fouling property.

Robust polylactide nanofibrous membranes by gelation/crystallization from solution.

Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.