Bovine Cluster of Differentiation 4 ELISA kit is suitable for the detection of samples from Bovine species. Cluster of Differentiation 4 can also be called as CD4 (L3T4); CD4 antigen (p55); CD4 Antigen ; CD4 molecule; CD4 Receptor; CD4+ Lymphocyte deficiency; included; CD4mut.
Specifications Of E11C0004 Bovine CD4 ELISA Kit
Product's Information | |
Cat. NO. | E11C0004 |
Product Name | Bovine Cluster of Differentiation 4 ELISA kit |
Species | Bovine |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50 ng/mL |
Sensitivity | 0.1 ng/mL |
Principal | Sandwich ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFIADPNION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 10 ng/mL | 1 vial |
STANDARD E (0.5mL) | 25 ng/mL | 1 vial |
STANDARD F (0.5mL) | 50 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
The CD4 ELISA kit uses an anti-CD4 antibody and a CD4-HRP conjugate to perform a competitive enzyme immunoassay. The CD4-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-CD4 antibody binding site between CD4 from samples and CD4-HRP conjugate, the intensity of the color is inversely proportional to the CD4 concentration. since CD4 from samples and CD4-HRP conjugate compete for the anti-CD4 antibody binding site. Since the number of sites is limited, as more sites are occupied by CD4 from the sample, fewer sites are left to bind CD4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD4 concentration in each sample is interpolated from this standard curve. |
Quality Control On Bovine CD4 ELISA Kit
CV | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery% | 90-105 | |
Linearity | Diluent ratio | Range % |
1:2 | 89-107 | |
1:4 | 85-108 | |
1:8 | 85-109 | |
Specificity/ Cross-reactivity | No significant cross-reactivity or interference between CD4 and analogues was observed. | |
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Summary Of The Assay Procedures For E11C0004 Bovine CD4 ELISA Kit
Citations Of Bovine Cluster Of Differentiation 4 ELISA Kit
To separation of lactic acid bacteria and yeast cultures for the application of cow and newborn calf perinatal care
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