Bovine Interleukin 6 ELISA kit - Shanghai BlueGene Biotech CO., LTD.

BlueGene Biotech Bovine Interleukin 6 ELISA kit

E11I0006 Bovine Interleukin 6 ELISA kit

The Bovine Interleukin 6 ELISA kit can be used to identify samples from the bovine species. Interleukin 6 can also be called Interleukin BSF 2, B cell differentiation factor, B cell stimulatory factor 2, BSF2, CDF, CTL differentiation factor, Cytotoxic T cell differentiation factor, Hepatocyte stimulating factor, HGF, HPGF, HSF, Hybridoma growth factor, Hybridoma plasmacytoma growth factor, IFNB2, IL6 protein, Interferon beta 2, Interleukin 6 (interferon beta 2), Interleukin 6, IL6, IL-6, IL 6.

Products

Specifications of Bovine Interleukin 6 ELISA kit

Product Information
Cat. No.	E11I0006
Product Name	Bovine Interleukin 6 ELISA kit
Species	Bovine
Product Size	48 Tests / 96 Tests
Concentration	50-1000 pg/ml
Sensitivity	1.0 pg/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 pg/mL	1 vial
STANDARD B (0.5mL)	50 pg/mL	1 vial
STANDARD C (0.5mL)	100 pg/mL	1 vial
STANDARD D (0.5mL)	250 pg/mL	1 vial
STANDARD E (0.5mL)	500 pg/mL	1 vial
STANDARD F (0.5mL)	1000 pg/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

IL6 ELISA kit uses an anti-IL6 antibody and an IL6-HRP conjugate in a competitive enzyme immunoassay method. IL6-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IL6 antibody binding site between IL6 from samples and IL6-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IL6. Since the number of sites is limited, as more sites are occupied by IL6 from the sample, fewer sites are left to bind IL6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL6 concentration in each sample is interpolated from this standard curve.

Quality Control on Bovine Interleukin 6 ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between IL6 and analogues was observed.