Canine Transforming Growth Factor β1 ELISA kit - Shanghai BlueGene Biotech CO., LTD.

BlueGene Biotech Canine Transforming Growth Factor β1 ELISA kit

E08T0009 Canine Transforming Growth Factor β1 ELISA kit

Canine Transforming Growth Factor β1 ELISA kit is suitable for the detection of samples from Canine species. Transforming Growth Factor β1 can also be called CED, DPD1, TGF beta 1, TGF beta, TGF beta 1 protein, TGF-beta 1 protein, TGF-beta-1, TGF-beta-5, TGF-beta1, TGFB, Tgfb-1, tgfb1, TGFbeta, TGFbeta1, Transforming Growth Factor b1, Transforming Growth Factor beta 1, Transforming growth factor beta 1a, transforming growth factor beta-1, transforming growth factor, beta 1, TGFβ1, TGF β1, TGF-β1.

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Specifications of Canine Transforming Growth Factor β1 ELISA kit

Product Information
Cat. No.	E08T0009
Product Name	Canine Transforming Growth Factor β1 ELISA kit
Species	Canine
Product Size	48 Tests / 96 Tests
Concentration	50-1000 pg/mL
Sensitivity	1.0 pg/mL
Principal	Sandwich ELISA
Sample Volume	50 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	10 mL	1 vial
STANDARD A (0.5mL)	0 pg/mL	1 vial
STANDARD B (0.5mL)	50 pg/mL	1 vial
STANDARD C (0.5mL)	100 pg/mL	1 vial
STANDARD D (0.5mL)	250 pg/mL	1 vial
STANDARD E (0.5mL)	500 pg/mL	1 vial
STANDARD F (0.5mL)	1000 pg/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

TGFβ1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for TGFβ1. Standards or samples are then added to the microtiter plate wells and TGFβ1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of TGFβ1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for TGFβ1 are added to each well to “sandwich” the TGFβ1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain TGFβ1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TGFβ1 concentration in each sample is interpolated from this standard curve.

Quality Control on Canine Transforming Growth Factor β1 ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	86-115%
Linearity	Diluent Ratio	Range %
	1:2	84-114
	1:4	87-117
	1:8	80-112
Specificity/Cross-reactivity	No significant cross-reactivity or interference between TGFβ1 and analogues was observed.