E12C0027 Chicken cyclic Adenosine Monophosphate ELISA kit
Chicken cyclic Adenosine Monophosphate ELISA kit is suitable for the detection of samples from Chicken species. Cyclic Adenosine Monophosphate can also be called cAMP, cyclic AMP, 3',5'-cyclic adenosine monophosphate.
Product Information | |
Cat. No. | E12C0027 |
Product Name | Chicken cyclic Adenosine Monophosphate ELISA kit |
Species | Chicken |
Product Size | 48 Tests / 96 Tests |
Concentration | 10-250pmol/mL |
Sensitivity | 1.0 pmol/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6 mL | 1 vial |
STANDARD A (0.5mL) | 0 pmol/mL | 1 vial |
STANDARD B (0.5mL) | 5.0 pmol/mL | 1 vial |
STANDARD C (0.5mL) | 10 pmol/mL | 1 vial |
STANDARD D (0.5mL) | 50 pmol/mL | 1 vial |
STANDARD E (0.5mL) | 100 pmol/mL | 1 vial |
STANDARD F (0.5mL) | 250 pmol/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
cAMP ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-cAMP antibody and an cAMP-HRP conjugate. The assay sample and buffer are incubated together with cAMP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the cAMP concentration since cAMP from samples and cAMP-HRP conjugate compete for the anti-cAMP antibody binding site. Since the number of sites is limited, as more sites are occupied by cAMP from the sample, fewer sites are left to bind cAMP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The cAMP concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 91-105% | |
Linearity | Diluent Ratio | Range % |
1:2 | 88-106 | |
1:4 | 87-108 | |
1:8 | 85-110 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between cAMP and analogues was observed. | |
E12C0027 has been referenced in the below publications:
Study on Acertain purity of traditional Chinese medicine compound polysaccharides with different MHC B-LβII genotype of chicken immunomodulatory effects receptor TLR2, TLR4 and Immune Signaling Molecule.
Effects of Osthole on Progesterone Secretion in Chicken Preovulatory Follicles Granulosa Cells.
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