E12N0011 Chicken Nuclear factor kB ELISA kit
Chicken Nuclear factor kB ELISA kit is suitable for the detection of samples from Chicken species. Nuclear factor kB can also be called NF-KB1 EBP-1 KBF1 NF-Kappa-B NFKB-P105 NFKB-P50 Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 1 Nuclear factor NF-kappa-B p105 subunit, NFkB.
Product Information | |
Cat. No. | E12N0011 |
Product Name | Chicken Nuclear factor kB ELISA kit |
Species | Chicken |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10 ng/mL |
Sensitivity | 0.1 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 0.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD E (0.5mL) | 5 ng/mL | 1 vial |
STANDARD F (0.5mL) | 10 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
NFkB ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-NFkB antibody and an NFkB-HRP conjugate. The assay sample and buffer are incubated together with NFkB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NFkB concentration since NFkB from samples and NFkB-HRP conjugate compete for the anti-NFkB antibody binding site. Since the number of sites is limited, as more sites are occupied by NFkB from the sample, fewer sites are left to bind NFkB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NFkB concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 91-105% | |
Linearity | Diluent Ratio | Range % |
1:2 | 88-106 | |
1:4 | 87-108 | |
1:8 | 85-110 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between NFkB and analogues was observed. | |
E12N0011 has been referenced in the below publications:
Study on Acertain purity of traditional Chinese medicine compound polysaccharides with different MHC B-LβII genotype of chicken immunomodulatory effects receptor TLR2, TLR4 and Immune Signaling Molecule.
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