E04C0234 Rabbit CD147/EMMPRIN ELISA kit
Rabbit CD147/EMMPRIN ELISA kit is suitable for detecting samples from rabbit species. CD147/EMMPRIN can also be called CD147, TCSF, Emmprin, 5A11 antigen, 5F7, Basigin, Blood brain barrier HT7 antigen, BSG, CD 147, CD147 antigen, Collagenase stimulatory factor, Extracellular matrix metalloproteinase inducer, Leukocyte activation antigen M6, M6, M6 leukocyte activation antigen, neurothelin,TCSF, Tumor cell derived collagenase stimulatory factor.
Product Information | |
Cat. No. | E04C0234 |
Product Name | Rabbit CD147/EMMPRIN ELISA kit |
Species | Rabbit |
Product Size | 48 Tests / 96 Tests |
Concentration | 100-2500pg/mL |
Sensitivity | 1.0 pg/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 100 pg/mL | 1 vial |
STANDARD C (0.5mL) | 250 pg/mL | 1 vial |
STANDARD D (0.5mL) | 500 pg/mL | 1 vial |
STANDARD E (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD F (0.5mL) | 2500 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
CD147 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- CD147 antibody and an CD147-HRP conjugate. The assay sample and buffer are incubated together with CD147-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CD147 concentration since CD147 from samples and CD147-HRP conjugate compete for the anti-CD147 antibody binding site. Since the number of sites is limited, as more sites are occupied by CD147 from the sample, fewer sites are left to bind CD147-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD147 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 89-114.79% | |
Linearity | Diluent Ratio | Range % |
1:2 | 96-116 | |
1:4 | 91-109 | |
1:8 | 88-103 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between CD147 and analogues was observed. | |
E04C0234 has been referenced in the below publications:
The study of stabilizing atherosclerotic vulnerable plaque by gene silencing CD147 using lentiviral-delivered small interference RNA.
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