E01D0004 Human Anti double stranded DNA ELISA kit
The Human Anti double stranded DNA ELISA kit can be used to identify samples from the human species. Anti double stranded DNA can also be called anti dsDNA, dsDNA-Ab.
Specifications of Human Anti double stranded DNA ELISA kit
Product Information | |
Cat. No. | E01D0004 |
Product Name | Human Anti double stranded DNA ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 25-500 IU/mL |
Sensitivity | 1.0 IU/mL |
Principal | Sandwich ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 10.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 IU/mL | 1 vial |
STANDARD B (0.5mL) | 25 IU/mL | 1 vial |
STANDARD C (0.5mL) | 50 IU/mL | 1 vial |
STANDARD D (0.5mL) | 100 IU/mL | 1 vial |
STANDARD E (0.5mL) | 250 IU/mL | 1 vial |
STANDARD F (0.5mL) | 500 IU/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
Anti-dsDNA ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with dsDNA antigen. Standards or samples are then added to the microtiter plate wells and anti-dsDNA if present, will bind to the antigen pre-coated wells. In order to quantitatively determine the amount of anti-dsDNA present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated anti-dsDNA antibody IgG is added to each well to “sandwich” the anti-dsDNA immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain Anti-dsDNA and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Anti-dsDNA concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Anti double stranded DNA ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between anti dsDNA and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Human Anti double stranded DNA ELISA kit
Citations of Human Anti double stranded DNA ELISA kit
E01D0004 has been referenced in the below publications：
DAMP production by human islets under low oxygen and nutrients in the presence or absence of an immunoisolating-capsule and necrostatin-1.
