E01G0341 Human Glucose Oxidase ELISA kit
The Human Glucose Oxidase ELISA kit can be used to identify samples from the human species. Glucose Oxidase can also be called GOx, GOD, notatin.
Specifications of Human Glucose Oxidase ELISA kit
Product Information | |
Cat. No. | E01G0341 |
Product Name | Human Glucose Oxidase ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 ug/ml |
Sensitivity | 1.0 ug/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ug/mL | 1 vial |
STANDARD B (0.5mL) | 50 ug/mL | 1 vial |
STANDARD C (0.5mL) | 100 ug/mL | 1 vial |
STANDARD D (0.5mL) | 250 ug/mL | 1 vial |
STANDARD E (0.5mL) | 500 ug/mL | 1 vial |
STANDARD F (0.5mL) | 1000 ug/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
GOD ELISA kit uses an anti-GOD antibody and an GOD-HRP conjugate in a competitive enzyme immunoassay method. GOD-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-GOD antibody binding site between GOD from samples and GOD-HRP conjugate, the intensity of the color is inversely proportional to the concentration of GOD. Since the number of sites is limited, as more sites are occupied by GOD from the sample, fewer sites are left to bind GOD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GOD concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Glucose Oxidase ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between GOD and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Human Glucose Oxidase ELISA kit
Citations of Human Glucose Oxidase ELISA kit
E01G0341 has been referenced in the below publications：
Study on the correlation of estrogen level and high-sensitivity C-reactive protein, blood hornocystein, fibrinogen in postmenopausal women with hypertension.
