E01I0010 Human Interleukin 1β ELISA kit
The Human Interleukin 1β ELISA kit can be used to identify samples from the human species. Interleukin 1β can also be called Catabolin, IL-1β, IL 1 beta, IL 1B, IL1B, IL-1, IL1-BETA, IL1F2, interleukin 1 beta, IL1beta, IL-1β, leukocytic pyrogen, leukocytic endogenous mediator, mononuclear cell factor, lymphocyte activating factorInterleukin-1 beta.
Product Information | |
Cat. No. | E01I0010 |
Product Name | Human Interleukin 1β ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
| IL 1β ELISA kit uses an anti-IL 1β antibody and a IL 1β-HRP conjugate in a competitive enzyme immunoassay method. IL 1β-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IL 1β antibody binding site between IL 1β from samples and IL 1β-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IL 1β. Since the number of sites is limited, as more sites are occupied by IL 1β from the sample, fewer sites are left to bind IL 1β-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL 1β concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between IL 1β and analogues was observed. | |
E01I0010 has been referenced in the below publications：
Adiponectin antagonises LPS-regulated secretion of inflammatory factors in airway epithelial cells, and its expression is regulated by many factors.
miR-143 inhibits interleukin-13-induced inflammatory cytokine and mucus production in nasal epithelial cells from allergic rhinitis patients by targeting IL13Rα1.
Safety of intravenous anesthesia with Propofol for oocyte retrieval in IVF-ET patients.
Coal tar pitch smoke extract-induced pyroptosis in human bronchial epithelial cells.
Influence of NLRP3, AIM2 and CASP1 genes in acute exacerbations of chronic obstructive pulmonary diseases.
Pyroptosis induced by zinc oxide nanoparticles in A549 cells.
Effect of Balanced Ultrafiltration on Plasma Concentration of Antimicrobial Agents and Inflammatory Factors during Cardiac Surgery.
Role of NLRP3 inflammasome in endothelial cells inflammation induced by high uric acid.
Effects of propofol on early postoperative cognitive function andinflammatory cytokines in the elderly.
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