E01N0011 Human Nuclear factor kB ELISA kit
The Human Nuclear factor kB ELISA kit can be used to identify samples from the human species. Nuclear factor kB can also be called NFkB, NF-KB1, EBP-1, KBF1, NF-Kappa-B, NFKB-P105, NFKB-P50, Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 1, Nuclear factor NF-kappa-B p105 subunit.
Product Information | |
Cat. No. | E01N0011 |
Product Name | Human Nuclear factor kB ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 ng/ml |
Sensitivity | 1.0 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 50 ng/mL | 1 vial |
STANDARD C (0.5mL) | 100 ng/mL | 1 vial |
STANDARD D (0.5mL) | 250 ng/mL | 1 vial |
STANDARD E (0.5mL) | 500 ng/mL | 1 vial |
STANDARD F (0.5mL) | 1000 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
NFkB ELISA kit uses an anti-NFkB antibody and an NFkB-HRP conjugate in a competitive enzyme immunoassay method. NFkB-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-NFkB antibody binding site between NFkB from samples and NFkB-HRP conjugate, the intensity of the color is inversely proportional to the concentration of NFkB. Since the number of sites is limited, as more sites are occupied by NFkB from the sample, fewer sites are left to bind NFkB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NFkB concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between NFkB and analogues was observed. | |
E01N0011 has been referenced in the below publications：
NF-kB activity of peripheral blood mononuclear cell,microinflammation,oxidative stress and cardiovascular disease in hemodialysis patients.
the relationship between nf-kb activity of peripheral blood mononuclear cell and cardiovascular disease in patients of maintenance hemodialysis.
Effect of combined treatment of phentolamine, nifedipine, and magnesium sulfate
on vascular endothelial function, nuclear factor κB, and plasma pentraxin 3 in patients with hypertensive disorder complicating pregnancy.
The relationship between NF-kB activity of peripheral blood mononuclear cell and cardiovascular disease(CVD) in patients of maintenance hemodialysis(MHD).
Abstract Section one:Plasma pentraxin3 in maintenance hemodialysis patients is associated with malnutrition and cardiovascular disease.
Study on the Effects of Angiotensin Ⅱ and Oxidized Low-density Lipoprotein on Toll like receptor 4 in Cultured Human monocyte Cells.
The Relationship Between NF-kB activity and PTX3 in the Peripheral Poly Mononuclear Cells in Patients with Pregnancy Induced Hypertension.
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