E01H0031 Human Total Homocystein ELISA kit
The Human Total Homocystein ELISA kit can be used to identify samples from the human species. Total Homocystein can also be called Homocysteine, Hcy.
Specifications of Human Total Homocystein ELISA kit
Product Information | |
Cat. No. | E01H0031 |
Product Name | Human Total Homocystein ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100 µmoL/L |
Sensitivity | 1.0 µmoL/L |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 µmoL/L | 1 vial |
STANDARD B (0.5mL) | 50 µmoL/L | 1 vial |
STANDARD C (0.5mL) | 100 µmoL/L | 1 vial |
STANDARD D (0.5mL) | 250 µmoL/L | 1 vial |
STANDARD E (0.5mL) | 500 µmoL/L | 1 vial |
STANDARD F (0.5mL) | 1000 µmoL/L | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
HCY ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-HCY antibody and an HCY-HRP conjugate. The assay sample and buffer are incubated together with HCY-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HCY concentration since HCY from samples and HCY-HRP conjugate compete for the anti-HCY antibody binding site. Since the number of sites is limited, as more sites are occupied by HCY from the sample, fewer sites are left to bind HCY-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HCY concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Total Homocystein ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between HCY and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Human Total Homocystein ELISA kit
Citations of Human Total Homocystein ELISA kit
E01H0031 has been referenced in the below publications：
ASSOCIATION OF THROMBOMODULIN GENE METHYLATION WITH THE ONSET OF CEREBRAL INFARCTION.
