E01A0045 Human Adeno Associated Virus type 2 ELISA Kit
The Human Adeno Associated Virus type 2 ELISA Kit can be used to identify samples from the human species. Adeno Associated Virus type 2 can also be called AAV2, Serotype 2.
Specifications of Human Adeno Associated Virus type 2 ELISA Kit
Product Information | |
Cat. No. | E01A0045 |
Product Name | Human Adeno Associated Virus type 2 ELISA Kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100 ug/ml |
Sensitivity | 1.0 ug/ml |
Principal | Sandwich ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 10.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ug/mL | 1 vial |
STANDARD B (0.5mL) | 5.0 ug/mL | 1 vial |
STANDARD C (0.5mL) | 10 ug/mL | 1 vial |
STANDARD D (0.5mL) | 25 ug/mL | 1 vial |
STANDARD E (0.5mL) | 50 ug/mL | 1 vial |
STANDARD F (0.5mL) | 100 ug/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
AAV2 ELISA kit uses an anti-AAV2 antibody and an AAV2-HRP conjugate in a competitive enzyme immunoassay method. AAV2-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-AAV2 antibody binding site between AAV2 from samples and AAV2-HRP conjugate, the intensity of the color is inversely proportional to the concentration of AAV2. Since the number of sites is limited, as more sites are occupied by AAV2 from the sample, fewer sites are left to bind AAV2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AAV2 concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Adeno Associated Virus type 2 ELISA Kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between AAV2 and analogues was observed. | |
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Summary of the Assay Procedure for Human Adeno Associated Virus type 2 ELISA Kit
Citations ofHuman Adeno Associated Virus type 2 ELISA Kit
E01A0045 has been referenced in the below publications：
Site-Specific PEGylated Adeno-Associated Viruses with Increased Serum Stability and Reduced Immunogenicity.
