E03S0003 Mouse Secretory Immunoglobulin A ELISA kit
The Mouse Secretory Immunoglobulin A ELISA kit can be used to identify samples from the mouse species. Secretory Immunoglobulin A can also be called SIgA, secretory IgA.
Specifications of Mouse Secretory Immunoglobulin A ELISA kit
Product Information | |
Cat. No. | E03S0003 |
Product Name | Mouse Secretory Immunoglobulin A ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 100-2500mg/L |
Sensitivity | 1.0mg/L |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 mg/L | 1 vial |
STANDARD B (0.5mL) | 100 mg/L | 1 vial |
STANDARD C (0.5mL) | 250 mg/L | 1 vial |
STANDARD D (0.5mL) | 500 mg/L | 1 vial |
STANDARD E (0.5mL) | 1000 mg/L | 1 vial |
STANDARD F (0.5mL) | 2500 mg/L | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
SIgA ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-SIgA antibody and an SIgA-HRP conjugate. The assay sample and buffer are incubated together with SIgA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SIgA concentration since SIgA from samples and SIgA-HRP conjugate compete for the anti-SIgA antibody binding site. Since the number of sites is limited, as more sites are occupied by SIgA from the sample, fewer sites are left to bind SIgA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SIgA concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Secretory Immunoglobulin A ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between SIgA and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Mouse Secretory Immunoglobulin A ELISA kit
Citations of Mouse Secretory Immunoglobulin A ELISA kit
E03S0003 has been referenced in the below publications：
Cryptosporidium parvum. Protective Immunity against Cryptosporidiosis with Recombinant Lactobacillus Live Vaccines Expressing cp966 after Oral Immunization.
Influence on gastric mucosa specific antibody in newborn mice after oral immunization with inactived H. pylori.
Study on the pathogenicity of UU1, UU3 and UU4 in the lower genital tract of female KM mice.
