E03U0078 Mouse Uric Acid ELISA kit
The Mouse Uric Acid ELISA kit can be used to identify samples from the mouse species. Uric Acid can also be called 2,6,8-Trioxypurine, 2,6,8-Trihydroxypurine, 2,6,8-Trioxopurine, 1H-Purine-2,6,8-trione, UA.
Specifications of Mouse Uric Acid ELISA kit
Product Information | |
Cat. No. | E03U0078 |
Product Name | Mouse Uric Acid ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 10-250umol/L |
Sensitivity | 1.0umol/L |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 umol/L | 1 vial |
STANDARD B (0.5mL) | 10 umol/L | 1 vial |
STANDARD C (0.5mL) | 25 umol/L | 1 vial |
STANDARD D (0.5mL) | 50 umol/L | 1 vial |
STANDARD E (0.5mL) | 100 umol/L | 1 vial |
STANDARD F (0.5mL) | 250 umol/L | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
UA ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-UA antibody and an UA-HRP conjugate. The assay sample and buffer are incubated together with UA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the UA concentration since UA from samples and UA-HRP conjugate compete for the anti-UA antibody binding site. Since the number of sites is limited, as more sites are occupied by UA from the sample, fewer sites are left to bind UA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The UA concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Uric Acid ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between UA and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Mouse Uric Acid ELISA kit
Citations of Mouse Uric Acid ELISA kit
E03U0078 has been referenced in the below publications：
Toll-like receptor 2-modulating pectin-polymers in alginate-based microcapsules attenuate immune responses and support islet-xenograft survival.
