E04S0222 Rabbit Soluble thrombomodulin ELISA kit
Rabbit Soluble thrombomodulin ELISA kit is suitable for detecting samples from rabbit species. Soluble thrombomodulin can also be called sTHBD, sAHUS6, sBDCA3, sCD141, sTHPH12, sTHRM, sthrombomodulin, sBDCA-3, sTM, s TM, s-TM.
Product Information | |
Cat. No. | E04S0222 |
Product Name | Rabbit Soluble thrombomodulin ELISA kit |
Species | Rabbit |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100ng/mL |
Sensitivity | 1.0 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 10 ng/mL | 1 vial |
STANDARD D (0.5mL) | 25 ng/mL | 1 vial |
STANDARD E (0.5mL) | 50 ng/mL | 1 vial |
STANDARD F (0.5mL) | 100 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
sTM ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- sTM antibody and an sTM-HRP conjugate. The assay sample and buffer are incubated together with sTM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the sTM concentration since sTM from samples and sTM-HRP conjugate compete for the anti-sTM antibody binding site. Since the number of sites is limited, as more sites are occupied by sTM from the sample, fewer sites are left to bind sTM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sTM concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 89-114.79% | |
Linearity | Diluent Ratio | Range % |
1:2 | 96-116 | |
1:4 | 91-109 | |
1:8 | 88-103 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between sTM and analogues was observed. | |
E04S0222 has been referenced in the below publications:
Comparison of two fluid solutions for resuscitation in a rabbit model of crush syndrome.
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