E02A0050 Rat Amyloid β Protein 42 ELISA kit
The Rat Amyloid β Protein 42 ELISA kit can be used to identify samples from the rat species. Amyloid β Protein 42 can also be called beta Amyloid(1-42), beta Amyloid 1-42, beta-Amyloid 1-42, Amyloid 1-42, beta-Amyloid 42, A4, AAA, ABETA, ABPP, AD1, Alzheimers Disease Amyloid Protein, Amyloid B, Amyloid Beta A4 Protein Precursor, Aβ42.
Product Information | |
Cat. No. | E02A0050 |
Product Name | Rat Amyloid β Protein 42 ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/mL |
Sensitivity | 1.0 pg/ml |
Principal | Sandwich ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 10 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/ml | 1 vial |
STANDARD B (0.5mL) | 50 pg/ml | 1 vial |
STANDARD C (0.5mL) | 100 pg/ml | 1 vial |
STANDARD D (0.5mL) | 250 pg/ml | 1 vial |
STANDARD E (0.5mL) | 500 pg/ml | 1 vial |
STANDARD F (0.5mL) | 1000 pg/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
Aβ42 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for Aβ42. Standards or samples are then added to the microtiter plate wells and Aβ42 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of Aβ42 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for Aβ42 are added to each well to “sandwich” the Aβ42 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain Aβ42 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Aβ42 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between Aβ42 and analogues was observed. | |
E02A0050 has been referenced in the below publications：
Alteration in amyloid β42, phosphorylated tau protein, interleukin 6, and acetylcholine during diabetes-accelerated memory dysfunction in diabetic rats: correlation of amyloid β42 with changes in glucose metabolism.
Roliram improved cognitive impairment and neuroinflammation in diabetic encephalopathy.
Protective effects of BDNF on hippocampal neurons under high glucose condition.
Roliram improved cognitive impairment and neuroinflammation in diabetic encephalopathy.
A study on correlation of cognitive function and expression with beta-amyloid，tumor necrosis factor alpha in hippocampus of diabetic rats.
Dynamic changes of major biomarkers in rats plasma during the development of diabete.
Expression of Arc/Arg3.1 in diabetic rat hippocampus and its relationship with cognitive function.
Alteration in amyloid β42,phosphorylated tau protein, interleukin 6,and acetylcholine during diabetes-accelerated memory dysfunction in diabetic rats: correlation of amyloid β42 with changes in glucose metabolismb.
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