E02A1946 Rat Apoptosis stimulating of p53 protein 1 ELISA kit
The Rat Apoptosis stimulating of p53 protein 1 ELISA kit can be used to identify samples from the rat species. Apoptosis stimulating of p53 protein 1 can also be called PPP1R13B, ASPP1, p53BP2-like, p85, protein phosphatase 1 regulatory subunit 13B.
Product Information | |
Cat. No. | E02A1946 |
Product Name | Rat Apoptosis stimulating of p53 protein 1 ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10 ng/ml |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 0.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD E (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD F (0.5mL) | 10 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
PPP1R13B ELISA kit uses an anti-PPP1R13B antibody and an PPP1R13B-HRP conjugate in a competitive enzyme immunoassay method. PPP1R13B-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-PPP1R13B antibody binding site between PPP1R13B from samples and PPP1R13B-HRP conjugate, the intensity of the color is inversely proportional to the concentration of PPP1R13B. Since the number of sites is limited, as more sites are occupied by PPP1R13B from the sample, fewer sites are left to bind PPP1R13B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PPP1R13B concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between PPP1R13B and analogues was observed. | |
E02A1946 has been referenced in the below publications：
糖尿病大鼠视网膜p53、p21WAF1/CIP1表达研究。
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