E02G0463 Rat G protein-coupled receptor kinase 2 ELISA kit
The Rat G protein-coupled receptor kinase 2 ELISA kit can be used to identify samples from the rat species. G protein-coupled receptor kinase 2 can also be called GRK2, BARK1, BETA, ARK1, ADRBK1, G protein-coupled receptor kinase 2, Beta-adrenergic receptor kinase.
Product Information | |
Cat. No. | E02G0463 |
Product Name | Rat G protein-coupled receptor kinase 2 ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10 ng/ml |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 0.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD E (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD F (0.5mL) | 10 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
GRK2 ELISA kit uses an anti-GRK2 antibody and an GRK2-HRP conjugate in a competitive enzyme immunoassay method. GRK2-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-GRK2 antibody binding site between GRK2 from samples and GRK2-HRP conjugate, the intensity of the color is inversely proportional to the concentration of GRK2. Since the number of sites is limited, as more sites are occupied by GRK2 from the sample, fewer sites are left to bind GRK2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GRK2 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between GRK2 and analogues was observed. | |
E02G0463 has been referenced in the below publications：
The Protective Effects and Mechanism of Ghrelin in Sepsis-induced Acute Kidney Injury.
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