CHO Host Cell Protein ELISA Kit, G1 is for research use only, it should not be used in clinical diagnostic procedures. The kit is intended for the quantitative determination of host cell protein impurities in Cell culture supernatants or host cell protein (HCP) contamination in end-product expressed by Chinese Hamster Ovary (CHO) expression systems.
Specifications Of CH-K0018-1 CHO Host Cell Protein ELISA Kit, G1
| Product's Information | |
| Cat. NO. | CH-K0018-1 |
| Products Name | CHO Host Cell Protein ELISA Kit, G1 |
| Species | |
| Product Size | 96 Tests |
| Concentration | 3.125-100ng/ml |
| Sensitivity | LOD: 0.2 ng/ml; LOQ: 3 ng/ml |
| Principal | Sandwich ELISA |
| Sample Volume | 100 ul |
| Sample Type | The kit is intended for the quantitative determination of host cell protein impurities in Cell culture supernatants or host cell protein (HCP) contamination in end-product expressed by Chinese Hamster Ovary (CHO) expression systems. |
| Assay Time | 3 hours and 10 minutes |
| Platform | Microplate Reader |
| Conjugate | HRP |
| Detection Method | Colorimetric |
| Storage | 2-8°C |
Kit Components | ||
Reagents | Specification | Quantity |
Pre-Coated Microplate (Detachable) | 96 wells | 1 plate (Keep Sealed) |
Standard (Stock Solution) -500ug/ml | 10ul | 1 tube |
Detection antibody (1400×) | 15ul | 1 tube |
TMB Substrates | 10ml | 1 vial (Avoid Light) |
Stop Solution | 10ml | 1 vial |
Wash Solution (100×) | 10ml | 1 vial |
Diluent Buffer (10×) | 10ml | 1 vial |
Diluent Ingredient | 0.5g | 1 tube |
Plate Sealer | 4 pieces | |
Instruction Manual | 1 | |
Principle of the Assay |
This ELISA kit is applied to the solid phase sandwich Enzyme Linked Immunosorbent Assay. The microtiter plate has been pre-coated with capture antibody. The antigen to be measured and the HRP-conjugated detection antibody are then added to the microtiter plate wells sequentially to form a capture antibody-antigen-detection antibody complex. After washing, the conjugates uninvolved in the reaction are removed, and then a TMB substrate solution is added to the wells to incubate. TMB substrate solution turns blue after the oxidation of HRP, and finally turns to yellow immediately after adding the stop solution. Color of TMB substrate positively correlated with the antigen bound in the initial steps. The color is measured by spectrophotometrically with wavelength of 450nm. The concentration of ErbB2 in samples is then determined by comparing the O.D. of the samples to the standard curve.
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Quality Control On CH-K0018-1 CHO Host Cell Protein Detection Kit, G1
CV | Intra Variation%: 3.1-4.5 | |
Inter Variation%: 6.0-9.1 | ||
Recovery% | 95-110 | |
Linearity | Diluent ratio | Range % |
1:2 | 98.9-103.3 | |
1:4 | 95.1-103 | |
1:8 | 93.5-104.5 | |
1:16 | 94.1-115.4 | |
1:32 | 100-109.4 | |
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Summary Of The Assay Procedures For CH-K0018-1 CHO Host Cell Protein ELISA Kit, G1
