E04E0182 Rabbit Total Endothelin ELISA kit
Rabbit Total Endothelin ELISA kit is suitable for detecting samples from rabbit species. Total Endothelin can also be called ET.
Specifications of Rabbit Total Endothelin ELISA kit
Product Information | |
Cat. No. | E04E0182 |
Product Name | Rabbit Total Endothelin ELISA kit |
Species | Rabbit |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/mL |
Sensitivity | 1.0 pg/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
ET ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- ET antibody and an ET-HRP conjugate. The assay sample and buffer are incubated together with ET-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ET concentration since ET from samples and ET-HRP conjugate compete for the anti-ET antibody binding site. Since the number of sites is limited, as more sites are occupied by ET from the sample, fewer sites are left to bind ET-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ET concentration in each sample is interpolated from this standard curve. |
Quality Control on Rabbit Total Endothelin ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 89-114.79% | |
Linearity | Diluent Ratio | Range % |
1:2 | 96-116 | |
1:4 | 91-109 | |
1:8 | 88-103 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between ET and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rabbit Total Endothelin ELISA kit
Citations of Rabbit Total Endothelin ELISA kit
E04E0182 has been referenced in the below publications:
The effect of nitriglycerin on the microcirculation disorder in the acute pancreatitis of rabbits.
Effect of extracorporeal shock wave on endothelin-1 expression and content in blood of glucocorticoid-induced femoral head necrosis in rabbits.
