Pichia Pastoris Host Cell Protein ELISA Kit, G3(One-Step Method) is for research use only, it should not be used in clinical diagnostic procedures.
This kit is intended for the quantitative detection of host cell protein (HCP) residues in biopharmaceuticals produced by Pichia Pastoris expression systems.
| Product's Information | |
| Cat. NO. | PH-E0021-3-48T |
| Products Name | Pichia Pastoris Host Cell Protein ELISA Kit, G3(One-Step Method) |
| Species | Pichia Pastoris |
| Product Size | 48 Tests |
| Concentration | 6.25-800ng/ml |
| Sensitivity | LOD: 1.56 ng/ml; LOQ: 6.25 ng/ml |
| Principal | Sandwich ELISA |
| Sample Volume | 100 ul |
| Sample Type | ELISA Kit for the quantitative Measurement of Pichia Pastoris HCP Residues in Culture Supernatants, Protein Purification Process, and End-Product |
| Assay Time | 3 hours and 15 minutes |
| Platform | Microplate Reader |
| Conjugate | HRP |
| Detection Method | Colorimetric |
| Storage | 2-8°C |
Kit Components | ||
Reagents | Specification | Quantity |
Pre-Coated Microplate (Detachable) | 48 wells | 1 plate (Keep Sealed) |
Standard (Stock Solution) -500ug/ml | 25ul | 1 tube |
Detection antibody (100×) | 75ul | 1 tube |
TMB Substrates | 10ml | 1 vial (Avoid Light) |
Stop Solution | 10ml | 1 vial |
Wash Solution (100×) | 10ml | 1 vial |
Diluent Buffer (10×) | 10ml | 1 vial |
Plate Sealer | 4 pieces | |
Instruction Manual | 1 | |
Principle of the Assay |
This ELISA kit is applied to the solid phase sandwich Enzyme-Linked Immunosorbent Assay. The microplate has been pre-coated with a capture antibody. HRP-conjugated detection antibody, standards, and the antigen to be measured are added simultaneously to the microplate pre-coated with capture antibody, allowing the formation of a capture antibody-antigen-detection antibody complex. After washing, the conjugates uninvolved in the reaction are removed, and then a TMB substrate solution is added to the wells to incubate. TMB substrate solution turns blue after the oxidation of HRP and finally turns yellow immediately after adding the stop solution. The color of the TMB substrate positively correlated with with the total concentration of host cell proteins (HCPs). Measure the absorbance (OD value) at 450nm using a microplate reader, and create a standard curve along with the corresponding concentrations. Then, by inputting the OD values of the samples into the standard curve equation, calculate the concentration of the target protein in the sample.
|
CV | Intra Variation%: 7-11 | |
Inter Variation%: 1.1-5.8 | ||
Linearity | Diluent ratio | Range % |
1:2 | 98.2-103.5 | |
1:4 | 95.2-102.6 | |
1:8 | 98.2-105.3 | |
1:16 | 95.3-104.1 | |
1:32 | 97.3-103.4 | |
| 1:64 | 88.8-103.2 | |
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