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Detection And Quality Control Of Host Proteins
Host proteins (HCP) form a major part of the process-related impurities in the production of biologics. The amount of residual HCP in a pharmaceutical product is often considered a critical quality attribute (CQA) because of its potential to affect product safety and efficacy.Aug.09, 2022Read More >
Definition And Structure Of Antibodies
Antibodies are shaped like the letter 'Y' and their main job is to bind to an invading antigen, but not to kill the antigen - they do not have this function; the binding is to give the antigen a marker to be destroyed. When phagocytes find the marker to destroy, they come forward and swallow the antibody-bound antigen and digest it.Aug.09, 2022Read More >
Selfish Bacterial Plasmids
Plasmids are a segment of circular DNA within a bacterium that is not part of the bacterial nuclear genome; they replicate within the cell and enter a daughter cell at random when it divides.Aug.09, 2022Read More >
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Horse ELISA Kits Types
E10A0530 Horse High Molecular Weight Adiponectin ELISA KitMANUAL
Cat. No.: E10A0530
Detection Range: 0.5-10 μg/mL
Method: Competitive ELISA
How to Prepare Feces Samples from Horse?
For the preparation of the feces samples, please find the steps below.
For one well, collect 300mg before homogenization. Mince the samples into small pieces and homogenized them in 500ul of PBS with a glass homogenizer on ice.
After that, the homogenates were centrifugated for 15 minutes at 1500×g (or 5000 rpm).
Collect the supernate and assay immediately or aliquot and store samples at -20°C or -80°C.
What Is the Difference between the Sensitivity and Lowest Detection Limit for Horse Elisa Kits?
Sensitivity and minimum detection limit are different concepts, the two are not equivalent.
Sensitivity is the smallest variation range of the kit to detect the difference change. This is calculated in the production process of the kit.
The lowest detection limit is measured as a high concentration of known samples, after N times dilution and calculating the CV of different dilutions until the CV exceeds the requirements of the general kit for CV, this concentration is the lowest detection limit of this kit.