CHO HCD Residue Detection Kits

During PCR amplification, the probe binds to the template along with the primers, with the probe located between the upstream and downstream primers. As the amplification reaches the position where the probe is bound, Taq polymerase cleaves the fluorescent molecule from the probe's 5' end using its 5' exonuclease activity, resulting in fluorescence emission. The detected fluorescence is proportional to the quantity of PCR products, and the initial DNA template amount can be calculated based on the fluorescence intensity in the PCR reaction system. TaqMan probe technology solves the problem of non-specific binding of fluorescent dyes during amplification, eliminating the need for melting curve analysis after the reaction, thus shortening the experimental time.


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Host Cell DNA Residue Detection Kits Types

Host Cell DNA Residue Detection Kits FAQs

  • Q

    What are the applications of Host Cell DNA Residue Detection Kits?

    The Host Cell DNA Residue Detection Kits are widely used for measuring residual amounts of host cell DNA in therapeutic antibody drugs.

  • Q

    What is the total Assay Time for Host Cell DNA Residue Detection Kit?

    The total assay time for the Host Cell DNA Residue Detection Kit depends on the amplification enzyme used in the kit and is independent of the target gene being detected. When using this kit, the entire amplification process takes no more than 1.5 hours.

  • Q

    What is the FDA limit for residual host cell DNA?

    The guidelines from the World Health Organization and the U.S. Food and Drug Administration suggest that the final product dose should contain no more than 10 ng of residual DNA and that the size of the residual DNA should be limited to 200 base pairs.

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