The plasmid is an extrachromosomal stable genetic factor, a closed-loop double-stranded DNA molecule, and is primarily present in the host cells in a supercoiled state.

Plasmids are mainly found in bacterial, actinomycete and fungal cells, capable of transcribing and replicating, they can be maintained at a constant copy number in progeny cells, and express the genetic information they carried.

Plasmids usually replicate under two type’s controls within cells: Stringent control of replication or relaxed control of replication. The former only replicate at a certain stage of the cell cycle, while the latter can replicate at any time throughout the cell cycle.

The replication and transcription of plasmids depend on certain enzymes and proteins encoded by the host cell, they would be unable to survive outside the host cell, but the host can survive normally without them.

Proteomes usually contain genes encoding certain enzymes, and their phenotypes include resistance to antibiotics, production of certain antibiotics, degradation of complex organic matter, production of colicins and enterotoxins, and certain restriction enzymes and modification enzymes.

Based on the natural plasmids, the plasmid vectors are constructed to adapt to laboratory operations. Compared with natural plasmids, plasmid vectors usually carry one or more selectable marker genes (such as antibiotic resistance genes) and a synthetic polyclonal sequence containing multiple restriction endonuclease recognition sites. , and removed most of the non-essential sequences, so as to reduce the weight as much as possible, and to facilitate genetic engineering operations.

  • Quality control—all our plasmids are 100% sequence verified.

  • Ready to use- an immediately available plasmid will assist you with your vector design and protein expression

  • Soluble expression

  • Good stability

  • Low cost

  • Wide application range

  • Highly expressive

  • Easy purification

Different Kinds Of BlueGene Biotech Plasmids

What are Plasmids and Their Functions?

What are Plasmids and Their Functions?
  • Plasmids, also known as plastids, refer to DNA molecules that can replicate autonomously outside the DNA of the cell's chromosomes or nuclear regions, and are not necessary for cell survival.

  • Most are circular molecules, a few are linear molecules, and many are found in prokaryotic bacteria, as well as in organisms such as yeast, or in plant mitochondrial organelles, which are much smaller than eukaryotic nuclear genomes, typically thousands to hundreds of thousands of base pairs. 

  • The gene carried by the plasmid can endow the cell with additional physiological and metabolic capabilities and improve its pathogenicity. The genetically engineered vector contains functional elements and can be replicated and transcribed independently.

What is the Difference Between DNA and Plasmids?

What is the Difference Between DNA and Plasmids?
  • DNA, comprising genomic DNA, organelle DNA, as well as plasmids, etc., all of which are polymerized from deoxyribonucleotides.

  • Nuclear DNA usually exists as chromosomes that replicate in equal amounts during cell division; 

  • Organelle DNA, such as mitochondrial DNA, is outside the nucleus of eukaryotes.It is cytoplasmic DNA, capable of semiautonomous self replication. It is usually more affected by the outside world, and encodes more mitochondrial functional proteins. 

  • Plasmids, such as the most common vector in genetic engineering, are circular DNAs that can independently exist in eukaryotic prokaryotic cells, contain functional elements, and can be independently replicated and transcribed.

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