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Rabbit ELISA Kits Types
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E04A0031 Rabbit Alkaline Phosphatase ELISA KitMANUAL
Cat. No.: E04A0031
Detection Range: 10-250ng/mL
Reactivity: Rabbit
Sensitivity: 1.0ng/mL
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E04A0204 Rabbit Angiotensin 2 ELISA KitMANUAL
Cat. No.: E04A0204
Detection Range: 50-1000pg/mL
Reactivity: Rabbit
Sensitivity: 1.0pg/mL
How To Obtain Rabbit Samples?
For domestic rabbit blood collection, there are usually several ways to take blood from the heart, ear marginal vein, ear central artery, femoral vein, jugular vein, etc. Fix the rabbit on its back to anesthetize, cut off the chest hair, disinfect the skin with iodophor, insert the needle on the skin of two-finger distance away from the top of its sternum, and draw out the whole blood. At the extreme edge of the back of the ear, wipe the skin with alcohol, pat it to expand the blood vessels, and insert the parallel needle in countercurrent. Use the same way for the ear central artery. When performing femoral vein surgery, the needle should be placed parallel to the lower end of the blood vessel, and hemostasis should be paid attention to. When taking blood from the jugular vein, it is needed to put the rabbit's head downward, cut off the fur, cut the skin, and then insert a needle to take blood.
Specimen Collection And Handling
For plasma, place the whole blood sample in the collection tube with anticoagulant, gently reverse and mix well, and then fully mix with the blood. The supernatant is the plasma sample after centrifugation at 3000 rpm for 10 min. For serum, it is lack fibrinogen compared with plasma. Collect the whole blood sample in a collection tube without anticoagulant, and put it at room temperature for 2-4 hours, or place it inclined at 4 ℃ in the refrigerator overnight. The blood coagulates and naturally precipitates serum, which can be centrifuged at 3000 rpm for 10 minutes to preserve. The serum and plasma are all light yellow and translucent liquid. When sampling, prevent the blood cells from breaking into the hemolytic state.