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Rabbit ELISA Kits

Rabbits are one of the most commonly used lab models in bioresearch and testing to study cardiovascular disease, skin conditions, and spinal cord injuries, and immune system, as well as asthma, cystic fibrosis, and diabetes etc. Among our more than 8000 Rabbit ELISA Kits, our premium rabbit ELISA Kits are suitable for serum, plasma, cell culture supernatants, body fluid and tissue homogenate. In cardiovascular disease, our Rabbit PDGF related ELISA kits are intended to assist the lab research, that due to PDGF pays an important role in blood vessel formation, the growth of blood vessels from already-existing blood vessel tissue, mitogenesis, i.e. proliferation, of mesenchymal cells such as fibroblasts, osteoblasts, tenocytes, vascular smooth muscle cells and mesenchymal stem cells as well as chemotaxis, the directed migration, of mesenchymal cells. 

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How To Obtain Rabbit Samples?

For domestic rabbit blood collection, there are usually several ways to take blood from the heart, ear marginal vein, ear central artery, femoral vein, jugular vein, etc. Fix the rabbit on its back to anesthetize, cut off the chest hair, disinfect the skin with iodophor, insert the needle on the skin of two-finger distance away from the top of its sternum, and draw out the whole blood. At the extreme edge of the back of the ear, wipe the skin with alcohol, pat it to expand the blood vessels, and insert the parallel needle in countercurrent. Use the same way for the ear central artery. When performing femoral vein surgery, the needle should be placed parallel to the lower end of the blood vessel, and hemostasis should be paid attention to. When taking blood from the jugular vein, it is needed to put the rabbit's head downward, cut off the fur, cut the skin, and then insert a needle to take blood.

Specimen Collection And Handling

For plasma, place the whole blood sample in the collection tube with anticoagulant, gently reverse and mix well, and then fully mix with the blood. The supernatant is the plasma sample after centrifugation at 3000 rpm for 10 min. For serum, it is lack fibrinogen compared with plasma. Collect the whole blood sample in a collection tube without anticoagulant, and put it at room temperature for 2-4 hours, or place it inclined at 4 ℃ in the refrigerator overnight. The blood coagulates and naturally precipitates serum, which can be centrifuged at 3000 rpm for 10 minutes to preserve. The serum and plasma are all light yellow and translucent liquid. When sampling, prevent the blood cells from breaking into the hemolytic state.

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