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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rabbit Tissue Factor ELISA kit

E04T0037 Rabbit Tissue Factor ELISA kit

Rabbit Tissue Factor ELISA kit is suitable for detecting samples from rabbit species. Tissue Factor can also be called Siderophilin, Serotransferrin, Beta-1 metal-binding globulin, TF.

Specifications of Rabbit Tissue Factor ELISA kit

Product Information

Cat. No.

E04T0037

Product Name

Rabbit Tissue Factor ELISA kit

Species

Rabbit

Product Size

48 Tests / 96 Tests

Concentration

100-2500pg/mL

Sensitivity

1.0 pg/mL

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

100 pg/mL

1 vial

STANDARD C (0.5mL)

250 pg/mL

1 vial

STANDARD D (0.5mL)

500 pg/mL

1 vial

STANDARD E (0.5mL)

1000 pg/mL

1 vial

STANDARD F (0.5mL)

2500 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

TF ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for TF. Standards or samples are then added to the microtiter plate wells and TF if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of TF present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for TF are added to each well to “sandwich” the TF immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain TF and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TF concentration in each sample is interpolated from this standard curve.


Quality Control on Rabbit Tissue Factor ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

89-114.79%

Linearity

Diluent Ratio

Range %

1:2

96-116

1:4

91-109

1:8

88-103

Specificity/Cross-reactivity

No significant cross-reactivity or interference between TF and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rabbit Tissue Factor ELISA kit

Summary of the Assay Procedure for Rabbit Tissue Factor ELISA kit

Citations of Rabbit Tissue Factor ELISA kit

E04T0037 has been referenced in the below publications:

To explore the inhibition of Polyinosinic acid combined statin on Cholesterol crystals as well as expression of hs-crp in atherosclerotic rabbits.

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