NE01I0038 Human Immunoglobulin M (IgM) ELISA Kit
This Kit is for the quantitative Measurement of Human Immunoglobulin M Residues in Serum, Plasma and Protein Purification Process, and End-Product (purified fermentation broth, cell culture supernatant, etc.)
Product Information | |
Cat. NO. | NE01I0038 |
Products Name | Human Immunoglobulin M (IgM) ELISA Kit |
Species | Human |
Product Size | 96 Tests |
Concentration | 0.78-50ng/mL |
Sensitivity | LOD: 0.19ng/mL; LOQ: 0.78ng/mL |
Principal | Sandwich ELISA |
Sample Volume | 100 ul |
Sample Type | ELISA Kit for the quantitative Measurement of Human Immunoglobulin M Residues in Serum, Plasma and Protein Purification Process, and End-Product (purified fermentation broth, cell culture supernatant, etc.) |
Assay Time | 3 hours and 15 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
Reagents | Specification | Quantity |
Pre-Coated Microplate (Detachable) | 96 wells | 1 plate (Keep Sealed) |
| Standard (Stock Solution) -10μg/mL | 10ul | 1 vial |
HRP-conjugated Detection antibody (100×) | 150μL | 1 vial |
| Diluent Buffer (10×) | 10mL | 1 via |
| TMB Substrates | 10ml | 1 vial (Avoid Light) |
Stop Solution | 10ml | 1 vial |
Wash Solution (20×) | 10ml | 5 vial |
| Plate Sealer | 4 pieces | |
Instruction Manual | 1 | |
Principle of the Assay |
This ELISA kit is applied to the double antibody sandwich Enzyme Linked Immunosorbent Assay to detect the concentration of human IgM in samples. The microtiter plate has been pre-coated with goat anti-human antibody, standards or samples are then added to the microtiter plate wells and IgM if present, will bind to the antibody pre-coated wells under specific conditions. Then wash the plate to remove unbound substances, add the HRP-conjugated goat anti-human antibody, and incubate at room temperature to form a double-antibody sandwich complex with the coated antibody, human IgM, and detection antibody. Then a TMB substrate solution is added to the wells to incubate. TMB substrate solution turns blue after the oxidation of HRP, and finally turns to yellow immediately after adding the stop solution. Color of TMB substrate positively correlated with total IgM bound in the initial steps. The color is measured by spectrophotometrically with wavelength of 450nm. The concentration of human IgM in samples is then determined by comparing the O.D. of the samples to the standard curve. |
Coefficient of Variance | Intra Variation:6-7% | ||
Inter Variation: 7-9% | |||
Specificity/ Cross-reactivity | Sample | Cross-Reactivity（%） | |
Mouse IgG | Not Detectable | ||
| Bovine IgG | Not Detectable | ||
| Human IgG | Not Detectable | ||
| Human IgM | Not Detectable | ||
Not available
Product: Not available
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