NE01I0004 Human Insulin (INS) ELISA Kit (Double Antibody Sandwich Method)
This kit is for the quantitative Measurement of Human Insulin in Serum, Plasma, Body Fluids and Tissue Homogenates.
Specifications of Human Insulin (INS) ELISA Kit (Double Antibody Sandwich Method)
Product Information | |
Cat. NO. | NE01I0004 |
Product Name | Human Insulin (INS) ELISA Kit (Double Antibody Sandwich Method) |
Species | Human |
Product Size | 96 Tests |
Concentration | 78.125-5000 pg/mL |
Sensitivity | LOD: 19.5pg/mL LOQ: 78.125pg/mL |
Principal | Sandwich ELISA |
Sample Volume | 100 ul |
Sample Type | ELISA Kit for the quantitative Measurement of Human Insulin in Serum, Plasma, Body Fluids and Tissue Homogenates |
Assay Time | 3 hours and 45 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
Reagents | Specification | Quantity |
Pre-Coated Microplate (Detachable) | 96 wells | 1 plate (Keep Sealed) |
| Standard (Stock Solution) -500ng/mL | 50μL | 1 vial |
HRP-conjugated Detection antibody(100×) | 150ul | 1 vial |
| TMB Substrates | 10ml | 1 vial (Avoid Light) |
Stop Solution | 10ml | 1 vial |
Wash Solution (100×) | 10ml | 1 vial |
| Diluent Buffer(10x) | 10ml | 1 vial |
Plate Sealer | 4 pieces | |
Instruction Manual | 1 | |
Principle of the Assay |
This ELISA kit is applied to the double antibody sandwich Enzyme Linked Immunosorbent Assay to detect the concentration of Insulin in samples. The microtiter plate has been pre-coated with anti-Insulin antibody, standards or samples are then added to the microtiter plate wells and Insulin if present, will bind to the antibody pre-coated wells under specific conditions. Then wash the plate to remove unbound substances, add the HRP-conjugated antibody, and incubate to form a double-antibody sandwich complex with the coated antibody, Insulin, and detection antibody. Then a TMB substrate solution is added to the wells to incubate. TMB substrate solution turns blue after the oxidation of HRP, and finally turns to yellow immediately after adding the stop solution. Color of TMB substrate positively correlated with total Insulin bound in the initial steps. The color is measured by spectrophotometrically with wavelength of 450nm. The concentration of Insulin in samples is then determined by comparing the O.D. of the samples to the standard curve. |
Quality Control on Human Insulin (INS) ELISA Kit (Double Antibody Sandwich Method)
Coefficient of Variance | Intra Variation%: 4-9 | ||
Inter Variation%: 2-7 | |||
| Recovery (%) | Spike Concentration | Recovery (%) | |
| Serum | 70-130 | ||
| Plasma | 70-130 | ||
| Cell supernatant | 80-120 | ||
| MCF-7 Complete Culture Medium | 85-120 | ||
BlueGene Biotech Product Show
Summary of the Assay Procedure for Human Insulin (INS) ELISA Kit (Double Antibody Sandwich Method)
Citations of Human Insulin (INS) ELISA Kit (Double Antibody Sandwich Method)
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