NEGEK0006 Kanamycin (KA) ELISA Kit (Two-Step Method). This Kit is for the quantitative Measurement of Kanamycin Residues in Samples such as Milk, Milk Powder, Tissue samples (Chicken, Pork).
Specifications of Kanamycin (KA) ELISA Kit (Two-Step Method)
Product Information | |
Cat. NO. | NEGEK0006 |
Products Name | Kanamycin (KA) ELISA Kit (Two-Step Method) |
Species | General |
Product Size | 96 Tests |
Concentration | 0-32ng/mL |
Sensitivity | 0.5ng/mL(ppb) |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | ELISA Kit for the quantitative Measurement of Kanamycin Residues in Samples such as Milk, Milk Powder, Tissue samples (Chicken, Pork) |
Assay Time | 3 hours and 15 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
Reagents | Specification | Quantity |
Pre-Coated Microplate (Detachable) | 96 wells | 1 plate (Keep Sealed) |
| Kanamycin Standard (Stock Solution) -6.4μg/mL | 20ul | 1 vial |
| Kanamycin Monoclonal Antibody (100×) | 100μL | 1 vial |
| HRP-conjugated Detection Antibody (100×) | 150μL | 1 vial |
| Diluent Buffer (10×) | 10mL | 1 vial |
| TMB Substrates | 10ml | 1 vial(Avoid Light) |
| Wash Solution (100 ×) | 10ml | 1 vial |
Stop Solution | 10ml | 1 vial |
| Plate Sealer | 4 pieces | |
Instruction Manual | 1 | |
Principle of the Assay |
This ELISA kit employs an indirect competitive immunoassay principle. The microplate is pre-coated with kanamycin, and standards (or samples) together with kanamycin monoclonal antibody are added simultaneously. The coated kanamycin antigen competes with kanamycin present in standards (or samples) for binding sites on the monoclonal antibody. After incubation at room temperature, unbound components are washed away. An HRP-conjugated secondary antibody is then added to bind to the captured kanamycin monoclonal antibody. A second incubation and wash step are performed to remove unbound substances. Finally, color development is carried out with TMB substrate, and the sample absorbance of the sample is negatively correlated with residual kanamycin concentration in the sample. The kanamycin concentration in the sample can be calculated by matching the absorbance value against the standard curve and multiplying by the corresponding dilution factor. |
Quality Control on Kanamycin (KA) ELISA Kit (Two-Step Method)
Coefficient of Variance | Intra Variation%:4-10.19 | ||
Inter Variation%: 7.68-9.96 | |||
| Recovery | Sample Type | Recovery Rate | |
| Milk | 80-120% | ||
| Milk Powder | 80-100% | ||
| Tissues | 80-120% | ||
Specificity/ Cross-reactivity | Sample | Cross-Reactivity | |
TOB (Tobramycin) | 37.79% | ||
NEO (Neomycin) | 0.02% | ||
STR (Streptomycin) | 0.01% | ||
GM (Gentamicin) | 0.08% | ||
BlueGene Biotech Product Show
Summary of the Assay Procedure for Kanamycin (KA) ELISA Kit (Two-Step Method)
Citations of Kanamycin (KA) ELISA Kit (Two-Step Method)
Not available
Product: Not available
