HH-H0019-3A1-Ab Anti-HEK293 Host Cell Protein G3 Antibody (HCP generated from conditioned media) has been validated by ELISA and 2D-WB methods, demonstrating high affinity for HCP, broad HCP coverage, and HEK293 cell specificity. It can be used for bridging experiments or quality control testing during production.
Specifications of HH-H0019-3A1-Ab Anti-HEK293 Host Cell Protein G3 Antibody (HCP generated from conditioned media）
| Product Information | |
Catalog Number | HH-H0019-3A1-Ab |
Package Size | 100μg/500ug |
| Type | Sheep polyclonal antibody IgG |
| Reactive Species | HEK293 |
| Purity | ≥95% |
| Physical Property | Liquid |
| Components | 0.01M PBS with 50% Glycerol, 0.3‰ Proclin300, sterile solution |
| Storage | -20℃. It is recommended to aliquot the product |
| Applications | ELISA, WB, Low-adsorption, High-throughput Protein Chip System, LC-MS |
| Coverage | ≥85% (validated by 2D-WB method) |
| Shelf Life | 5 Years |
Product Introduction |
This antibody is intended for the quantitative detection of host cell protein (HCP) residues in biopharmaceuticals produced using 293cell (HEK293) as the expression systems. 293 cell lines are widely utilized for the production of therapeutic proteins. During target protein expression, inherent cellular apoptosis occurs concomitantly. Published data indicate that over one thousand distinct host cell proteins are released into the culture medium upon cell lysis. A substantial proportion of these proteins exhibit strong immunogenicity, which can trigger adverse toxic or immune responses. Such responses jeopardize the safety and quality of biopharmaceutical products while posing risks of potential biological contamination. Consequently, the removal of these hazardous impurities represents a core objective of downstream biomanufacturing processes. Therefore, it is imperative to minimize residual HCP levels to the lowest feasible extent. In the development of downstream purification processes, a scientifically validated and reliable method for quantifying HCP concentrations in both finished and semi-finished products is indispensable. Enzyme-Linked Immunosorbent Assay (ELISA), renowned for its ultra-high sensitivity, has been established as the gold standard for HCP detection by regulatory authorities worldwide, including the FDA, EMA, NMPA, and ICH. This antibody has been validated by ELISA and 2D-WB methods, demonstrating high affinity for HCP, broad HCP coverage, and HEK293 cell specificity. It can be used for bridging experiments or quality control testing during production. |
