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BlueGene Biotech Human P53 ELISA kit (E01P0041)

E01P0041 Human P53 ELISA kit

The Human P53 ELISA kit can be used to identify samples from the human species. P53 can also be called TP53, LFS1, TRP53, Li-Fraumeni Syndrome, Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53.

Products

Specifications of Human P53 ELISA kit

Product Information

Cat. No.

E01P0041

Product Name

Human P53 ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

2.5-50ng/mL

Sensitivity

0.1 ng/ml

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5.0 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

P53 ELISA kit uses an anti-P53 antibody and an P53-HRP conjugate in a competitive enzyme immunoassay method. P53-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-P53 antibody binding site between P53 from samples and P53-HRP conjugate, the intensity of the color is inversely proportional to the concentration of P53. Since the number of sites is limited, as more sites are occupied by P53 from the sample, fewer sites are left to bind P53-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The P53 concentration in each sample is interpolated from this standard curve.


Quality Control on Human P53 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between P53 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human P53 ELISA kit

Summary of the Assay Procedure for Human P53 ELISA kit

Citations of Human P53 ELISA kit

E01P0041 has been referenced in the below publications:

Association between Polycyclic Aromatic Hydrocarbons and DNA damage serum levels of P53 and P21 proteins in neonates from an E-waste Recycling Area.


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