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  • ne01i0431 human immunoglobulin g elisa kit
  • ne01i0431 human immunoglobulin g elisa kit

BlueGene Biotech Human Immunoglobulin G ELISA Kit (NE01I0431)

NE01I0431 Human Immunoglobulin G ELISA Kit

Human Immunoglobulin G ELISA kit is suitable for the detection of samples from human species. Immunoglobulin G can also be called as IgG.

Products

Specifications of Human Immunoglobulin G ELISA kit

Product Information

Cat. NO.

NE01I0431

Products Name

Human Immunoglobulin G ELISA kit

Species

Human

Product Size

96 Tests

Concentration

156.25-10000 pg/ml

Sensitivity

10.2 pg/ml

Principal

Sandwich ELISA

Sample Volume

100 ul

Sample Type

ELISA Kit for the quantitative Measurement of human IgG Residues in Protein Purification

Process, and End-Product (purified fermentation broth, cell culture supernatant, etc.)

Assay Time

3 hours and 15 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C



Kit Components

Reagents

Specification

Quantity

Pre-Coated Microplate (Detachable)

96 wells

1 plate (Keep Sealed)

Standard (Stock Solution) -5ug/ml20ul1 vial

Standard S1

10000pg/ml

1 vial

Standard S2

5000pg/ml

1 vial

Standard S3

2500pg/ml

1 vial

Standard S4

1250pg/ml

1 vial

Standard S5

625pg/ml

1 vial

Standard S6

312.5pg/ml

1 vial

Standard S7

156.25pg/ml

1 vial

Standard S80pg/ml1 vial

Detection antibody (100×)

150ul

1 vial

HRP-conjugated antibody(200×)

100ul

1 vial

Diluent Buffer (10×)10ml

1 vial

TMB Substrates

10ml

1 vial (Avoid Light)

Stop Solution

10ml

1 vial

Wash Solution (100×)

10ml

1 vial

Plate Sealer


4 pieces

Instruction Manual


1


Principle of the Assay

This ELISA kit is applied to the double antibody sandwich Enzyme Linked Immunosorbent Assay to detect the concentration of human IgG in samples. The microtiter plate has been pre-coated with goat anti-human antibody , standards or samples are then added to the microtiter plate wells and IgG if present, will bind to the antibody pre-coated wells under specific conditions. After washing, then a coupled biotin-conjugated detection goat anti-human antibody is added to the wells, under 37℃ incubation, to form a precoated antibody-human IgG-detection antibody sandwich complex. After washing, coupled avidin conjugated Horseradish Peroxidase (HRP) is added to the wells, resulting in a cascade amplification effect. Then a TMB substrate solution is added to the wells to incubate. TMB substrate solution turns blue after the oxidation of HRP, and finally turns to yellow immediately after adding the stop solution. Color of TMB substrate positively correlated with total IgG bound in the initial steps. The color is measured by spectrophotometrically with wavelength of 450nm. The concentration of human IgG in samples is then determined by comparing the O.D. of the samples to the standard curve


Quality Control on Human Immunoglobulin G ELISA kit

Coefficient of Variance

Intra Variation: 2.7-3.14%

Inter Variation: 8.4-9.6%

Recovery

88.77-113.22%

Linearity

Diluent Ratio

Range %

Average Linearity %

1

97.43-98.31

97.87

1:2

103.01-103.30

103.15

1:4

97.23-102.06

99.65

1:8

89.02-101.74

95.38

1:16

99.86-114.11

106.98

1:32

101.25-105.87

103.56

1:64

80.07-96.14

88.11

Specificity/

Cross-reactivity

Sample

Cross-Reactivity(%)

Mouse IgG

0.349

Bovine IgG

---

VERO HCP

---

293 HCP

---

MDCK HCP

---

OVA

---

chicken anti-SPA

---

TRF

---


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Immunoglobulin G ELISA kit

Summary of the Assay Procedure for Human Immunoglobulin G ELISA kit

Citations of Human Immunoglobulin G ELISA kit

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