NE01I0431 Human Immunoglobulin G ELISA Kit
Human Immunoglobulin G ELISA kit is suitable for the detection of samples from human species. Immunoglobulin G can also be called as IgG.
Specifications of Human Immunoglobulin G ELISA kit
Product Information | |
Cat. NO. | NE01I0431 |
Products Name | Human Immunoglobulin G ELISA kit |
Species | Human |
Product Size | 96 Tests |
Concentration | 156.25-10000 pg/ml |
Sensitivity | 10.2 pg/ml |
Principal | Sandwich ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 3 hours and 15 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
Reagents | Specification | Quantity |
Pre-Coated Microplate (Detachable) | 96 wells | 1 plate (Keep Sealed) |
Standard S1 | 10000pg/ml | 1 vial |
Standard S2 | 5000pg/ml | 1 vial |
Standard S3 | 2500pg/ml | 1 vial |
Standard S4 | 1250pg/ml | 1 vial |
Standard S5 | 625pg/ml | 1 vial |
Standard S6 | 312.5pg/ml | 1 vial |
Standard S7 | 156.25pg/ml | 1 vial |
| Standard S8 | 0pg/ml | 1 vial |
Detection antibody (25×) | 500ul | 1 vial |
Detection antibody Diluent | 10.5ml | 1 vial |
HRP-Streptavidin (HRP, 200×) | 80ul | 1 vial |
HRP-Streptavidin Diluent | 10.5ml | 1 vial |
TMB Substrates | 10.5ml | 1 vial (Avoid Light) |
Stop Solution | 6ml | 1 vial |
Wash Solution (100×) | 10ml | 1 vial |
Plate Sealer | 4 pieces | |
Instruction Manual | 1 | |
Principle of the Assay |
This ELISA kit is applied to the double antibody sandwich Enzyme Linked Immunosorbent Assay to detect the concentration of human IgG in samples. The microtiter plate has been pre-coated with goat anti-human antibody , standards or samples are then added to the microtiter plate wells and IgG if present, will bind to the antibody pre-coated wells under specific conditions. After washing, then a coupled biotin-conjugated detection goat anti-human antibody is added to the wells, under 37℃ incubation, to form a precoated antibody-human IgG-detection antibody sandwich complex. After washing, coupled avidin conjugated Horseradish Peroxidase (HRP) is added to the wells, resulting in a cascade amplification effect. Then a TMB substrate solution is added to the wells to incubate. TMB substrate solution turns blue after the oxidation of HRP, and finally turns to yellow immediately after adding the stop solution. Color of TMB substrate positively correlated with total IgG bound in the initial steps. The color is measured by spectrophotometrically with wavelength of 450nm. The concentration of human IgG in samples is then determined by comparing the O.D. of the samples to the standard curve |
Quality Control on Human Immunoglobulin G ELISA kit
Coefficient of Variance | Intra Variation: 2.7-3.14% | ||
Inter Variation: 8.4-9.6% | |||
Recovery | 88.77-113.22% | ||
Linearity | Diluent Ratio | Range % | Average Linearity % |
1 | 97.43-98.31 | 97.87 | |
1:2 | 103.01-103.30 | 103.15 | |
1:4 | 97.23-102.06 | 99.65 | |
1:8 | 89.02-101.74 | 95.38 | |
1:16 | 99.86-114.11 | 106.98 | |
1:32 | 101.25-105.87 | 103.56 | |
1:64 | 80.07-96.14 | 88.11 | |
Specificity/ Cross-reactivity | Sample | Cross-Reactivity（%） | |
Mouse IgG | 0.349 | ||
Bovine IgG | --- | ||
VERO HCP | --- | ||
293 HCP | --- | ||
MDCK HCP | --- | ||
OVA | --- | ||
chicken anti-SPA | --- | ||
TRF | --- | ||
BlueGene Biotech Product Show
Summary of the Assay Procedure for Human Immunoglobulin G ELISA kit
Citations of Human Immunoglobulin G ELISA kit
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