E04S0012 Rabbit Superoxide Dismutase ELISA kit
Rabbit Superoxide Dismutase ELISA kit is suitable for detecting samples from rabbit species. Superoxide Dismutase can also be called SOD1, Cu-Zn SOD, Ipo1, IPOA, SOD, cytosolic, SOD, Soluble, ALS, ALS1, amyotrophic lateral sclerosis 1, adult, Cu, Zn superoxide dismutase, hSod1, indophenoloxidase A, IPOA, SOD, Superoxide dismutase 1, superoxide dismutase 1, soluble, superoxide dismutase, cystolic.
Product Information | |
Cat. No. | E04S0012 |
Product Name | Rabbit Superoxide Dismutase ELISA kit |
Species | Rabbit |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25ug/mL |
Sensitivity | 0.1 ug/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ug/mL | 1 vial |
STANDARD B (0.5mL) | 1.0 ug/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ug/mL | 1 vial |
STANDARD D (0.5mL) | 5.0 ug/mL | 1 vial |
STANDARD E (0.5mL) | 10 ug/mL | 1 vial |
STANDARD F (0.5mL) | 25 ug/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
SOD ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- SOD antibody and an SOD-HRP conjugate. The assay sample and buffer are incubated together with SOD-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SOD concentration since SOD from samples and SOD-HRP conjugate compete for the anti-SOD antibody binding site. Since the number of sites is limited, as more sites are occupied by SOD from the sample, fewer sites are left to bind SOD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SOD concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 89-114.79% | |
Linearity | Diluent Ratio | Range % |
1:2 | 96-116 | |
1:4 | 91-109 | |
1:8 | 88-103 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between SOD and analogues was observed. | |
E04S0012 has been referenced in the below publications:
The effects of ultrasonic therapy on osteoarthritis in knee of rabbits.
The Study about Mechanism of Onion stalk extraction on Prevention and Treatment of Rabbits’Traumatic Deep Vein Thrombosis.
Influences of Welsh-onion Stalk Extract on SOD and MDA in Formation of Traumatic Thrambosis on Deep Vein of Lower Limb in Rabbit.
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