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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rabbit N terminal pro brain natriuretic peptide ELISA kit

E04N0008 Rabbit N terminal pro brain natriuretic peptide ELISA kit

Rabbit N terminal pro brain natriuretic peptide ELISA kit is suitable for detecting samples from rabbit species. N terminal pro brain natriuretic peptide can also be called BNPT, NT proBNP.

Specifications of Rabbit N terminal pro brain natriuretic peptide ELISA kit

Product Information

Cat. No.

E04N0008

Product Name

Rabbit N terminal pro brain natriuretic peptide ELISA kit

Species

Rabbit

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/mL

Sensitivity

1.0 pg/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

NT proBNP ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- NT proBNP antibody and an NT proBNP-HRP conjugate. The assay sample and buffer are incubated together with NT proBNP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NT proBNP concentration since NT proBNP from samples and NT proBNP-HRP conjugate compete for the anti-NT proBNP antibody binding site. Since the number of sites is limited, as more sites are occupied by NT proBNP from the sample, fewer sites are left to bind NT proBNP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NT proBNP concentration in each sample is interpolated from this standard curve.


Quality Control on Rabbit N terminal pro brain natriuretic peptide ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

89-114.79%

Linearity

Diluent Ratio

Range %

1:2

96-116

1:4

91-109

1:8

88-103

Specificity/Cross-reactivity

No significant cross-reactivity or interference between NT proBNP and analogues was observed.


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Summary of the Assay Procedure for Rabbit N terminal pro brain natriuretic peptide ELISA kit

Summary of the Assay Procedure for Rabbit N terminal pro brain natriuretic peptide ELISA kit

Citations of Rabbit N terminal pro brain natriuretic peptide ELISA kit

E04N0008 has been referenced in the below publications:

Sacubitril/valsartan attenuates atrial electrical and structural remodelling in a rabbit model of atrial fibrillation.

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