E04A0644 Rabbit Acyl CoA synthetase ELISA kit
Rabbit Acyl CoA synthetase ELISA kit is suitable for detecting samples from rabbit species. Acyl CoA synthetase can also be called ACS, Acetyl-CoA synthetase, Acetate-CoA ligase, ACS.
Specifications of Rabbit Acyl CoA synthetase ELISA kit
Product Information | |
Cat. No. | E04A0644 |
Product Name | Rabbit Acyl CoA synthetase ELISA kit |
Species | Rabbit |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100ng/mL |
Sensitivity | 1.0 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 10 ng/mL | 1 vial |
STANDARD D (0.5mL) | 25 ng/mL | 1 vial |
STANDARD E (0.5mL) | 50 ng/mL | 1 vial |
STANDARD F (0.5mL) | 100 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
ACS ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- ACS antibody and an ACS-HRP conjugate. The assay sample and buffer are incubated together with ACS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACS concentration since ACS from samples and ACS-HRP conjugate compete for the anti-ACS antibody binding site. Since the number of sites is limited, as more sites are occupied by ACS from the sample, fewer sites are left to bind ACS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACS concentration in each sample is interpolated from this standard curve. |
Quality Control on Rabbit Acyl CoA synthetase ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 89-114.79% | |
Linearity | Diluent Ratio | Range % |
1:2 | 96-116 | |
1:4 | 91-109 | |
1:8 | 88-103 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between ACS and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rabbit Acyl CoA synthetase ELISA kit
Citations of Rabbit Acyl CoA synthetase ELISA kit
E04A0644 has been referenced in the below publications:
To explore the inhibition of Polyinosinic acid combined statin on Cholesterol crystals as well as expression of hs-crp in atherosclerotic rabbits.
