Rabbit Hepatocyte Growth Factor ELISA kit - Shanghai BlueGene Biotech CO., LTD.

BlueGene Biotech Rabbit Hepatocyte Growth Factor ELISA kit

E04H0208 Rabbit Hepatocyte Growth Factor ELISA kit

Rabbit Hepatocyte Growth Factor ELISA kit is suitable for detecting samples from rabbit species. Hepatocyte Growth Factor can also be called HGF, HGF alpha, HGFA, F TCF, Hepatocyte growth factor, Fibroblast derived tumor cytotoxic factor, Hepatocyte growth factor precursor, Hepatopoietin A, HPTA, HGF.

Products

Specifications of Rabbit Hepatocyte Growth Factor ELISA kit

Product Information
Cat. No.	E04H0208
Product Name	Rabbit Hepatocyte Growth Factor ELISA kit
Species	Rabbit
Product Size	48 Tests / 96 Tests
Concentration	1.0-25ng/mL
Sensitivity	0.1 ng/mL
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	1.0 ng/mL	1 vial
STANDARD C (0.5mL)	2.5 ng/mL	1 vial
STANDARD D (0.5mL)	5.0 ng/mL	1 vial
STANDARD E (0.5mL)	10 ng/mL	1 vial
STANDARD F (0.5mL)	25 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

HGF ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- HGF antibody and an HGF-HRP conjugate. The assay sample and buffer are incubated together with HGF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HGF concentration since HGF from samples and HGF-HRP conjugate compete for the anti-HGF antibody binding site. Since the number of sites is limited, as more sites are occupied by HGF from the sample, fewer sites are left to bind HGF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HGF concentration in each sample is interpolated from this standard curve.

Quality Control on Rabbit Hepatocyte Growth Factor ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	89-114.79%
Linearity	Diluent Ratio	Range %
	1:2	96-116
	1:4	91-109
	1:8	88-103
Specificity/Cross-reactivity	No significant cross-reactivity or interference between HGF and analogues was observed.