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  • bovine serum albumin elisa 1

BlueGene Biotech Rabbit Stromelysin-2 ELISA (E04S0299)

E04S0299 Rabbit Stromelysin-2 ELISA

Rabbit Stromelysin-2 ELISA is suitable for detecting samples from rabbit species. Stromelysin-2 can also be called MMP-10, MMP10, SL-2, STMY2, Stromelysin-2, Transin-2, ST2, ST 2, ST-2.

Products

Rabbit Stromelysin-2 ELISA

Product Information

Cat. No.

E04S0299

Product Name

Rabbit Stromelysin-2 ELISA

Species

Rabbit

Product Size

48 Tests / 96 Tests

Concentration

0.5-10ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

0.5 ng/mL

1 vial

STANDARD C (0.5mL)

1.0 ng/mL

1 vial

STANDARD D (0.5mL)

2.5 ng/mL

1 vial

STANDARD E (0.5mL)

5.0 ng/mL

1 vial

STANDARD F (0.5mL)

10 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

ST2 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- ST2 antibody and an ST2-HRP conjugate. The assay sample and buffer are incubated together with ST2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ST2 concentration since ST2 from samples and ST2-HRP conjugate compete for the anti-ST2 antibody binding site. Since the number of sites is limited, as more sites are occupied by ST2 from the sample, fewer sites are left to bind ST2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ST2 concentration in each sample is interpolated from this standard curve.


Quality Control on Rabbit Stromelysin-2 ELISA

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

89-114.79%

Linearity

Diluent Ratio

Range %

1:2

96-116

1:4

91-109

1:8

88-103

Specificity/Cross-reactivity

No significant cross-reactivity or interference between ST2 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rabbit Stromelysin-2 ELISA

Summary of the Assay Procedure for Rabbit Stromelysin-2 ELISA

Citations of Rabbit Stromelysin-2 ELISA

E04S0299 has been referenced in the below publications:

Sacubitril/valsartan attenuates atrial electrical and structural remodelling in a rabbit model of atrial fibrillation.

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