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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rabbit Tumor Necrosis Factor Alpha ELISA kit

E04T0008 Rabbit Tumor Necrosis Factor Alpha ELISA kit

Rabbit Tumor Necrosis Factor Alpha ELISA kit is suitable for detecting samples from rabbit species. Tumor Necrosis Factor Alpha can also be called DIF, TNF-A, TNFSF2, Cachectin, Tumor Necrosis Factor Ligand Superfamily Member 2, TNFAlpha, TNF a, TNF-a, TNFa.

Specifications of Rabbit Tumor Necrosis Factor Alpha ELISA kit

Product Information

Cat. No.

E04T0008

Product Name

Rabbit Tumor Necrosis Factor Alpha ELISA kit

Species

Rabbit

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/mL

Sensitivity

1.0 pg/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

TNFa ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- TNFa antibody and an TNFa-HRP conjugate. The assay sample and buffer are incubated together with TNFa-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TNFa concentration since TNFa from samples and TNFa-HRP conjugate compete for the anti-TNFa antibody binding site. Since the number of sites is limited, as more sites are occupied by TNFa from the sample, fewer sites are left to bind TNFa-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TNFa concentration in each sample is interpolated from this standard curve.


Quality Control on Rabbit Tumor Necrosis Factor Alpha ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

89-114.79%

Linearity

Diluent Ratio

Range %

1:2

96-116

1:4

91-109

1:8

88-103

Specificity/Cross-reactivity

No significant cross-reactivity or interference between TNFa and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rabbit Tumor Necrosis Factor Alpha ELISA kit

Summary of the Assay Procedure for Rabbit Tumor Necrosis Factor Alpha ELISA kit

Citations of Rabbit Tumor Necrosis Factor Alpha ELISA kit

E04T0008 has been referenced in the below publications:

TIMP‑3 suppresses the proliferation and migration of SMCs from the aortic neck of atherosclerotic AAA in rabbits, via decreased MMP‑2 and MMP‑9 activity, and reduced TNF‑α expression.

The effects and mechanism ofbone marrow mesenchymal stem cells transplantation on the stability of vulnerable plaque.

The effets of microwave on osteoarthritis in knee of rabbits.

The impact of Ultrasound-guided cryoablation on the local scarring and inflammation and it's application in breast benign lesions.

Effect of ischemic postconditioning on NF-kB activation signaling pathway in early lung transplantation.

The Experimental Study of Perfluorocarbon Pretreatment Intratracheally in Rabbit Model of Acute Lung Injury.

Effect of necrotic wound tissue decomposition products on serum inflammation factors in rabbits.

Effects of xilei oral sustained-release mucoadhesive films on expression of TNF- a, IL-1 in RAU rabbits models.

The Experimental Study of Pretreatment Doses of Perfluorocarbon in Acute Lung Injury Rabbit.

Transplantation of human umbilical cord mesenchymal stem cells in the treatment of systemic lupus erythematosus.

N-acetylcysteine advancement of surfactant therapy in experimental meconium aspiration syndrome: possible mechanisms.

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