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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rabbit Osteopontin ELISA kit (E04O0005)

E04O0005 Rabbit Osteopontin ELISA kit

Rabbit Osteopontin ELISA kit is suitable for detecting samples from rabbit species. Osteopontin can also be called SPP1, BNSP, BSPI, ETA1, Secreted Phosphoprotein 1, Bone Sialoprotein I, Early T-Lymphocyte Activation 1, Nephropontin, Urinary stone protein, Uropontin, OPN.

Products

Specifications of Rabbit Osteopontin ELISA kit

Product Information

Cat. No.

E04O0005

Product Name

Rabbit Osteopontin ELISA kit

Species

Rabbit

Product Size

48 Tests / 96 Tests

Concentration

2.5-50ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5.0 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

OPN ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- OPN antibody and an OPN-HRP conjugate. The assay sample and buffer are incubated together with OPN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the OPN concentration since OPN from samples and OPN-HRP conjugate compete for the anti-OPN antibody binding site. Since the number of sites is limited, as more sites are occupied by OPN from the sample, fewer sites are left to bind OPN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OPN concentration in each sample is interpolated from this standard curve.


Quality Control on Rabbit Osteopontin ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

89-114.79%

Linearity

Diluent Ratio

Range %

1:2

96-116

1:4

91-109

1:8

88-103

Specificity/Cross-reactivity

No significant cross-reactivity or interference between OPN and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rabbit Osteopontin ELISA kit

Summary of the Assay Procedure for Rabbit Osteopontin ELISA kit

Citations of Rabbit Osteopontin ELISA kit

E04O0005 has been referenced in the below publications:

Antibacterial, angiogenic, and osteogenic activities of ca, p, co, f, and Sr compound doped titania coatings with different Sr content.

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