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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rabbit 5 Hydroxytryptamine ELISA kit (E04H0106)

E04H0106 Rabbit Serotonin/5 Hydroxytryptamine ELISA kit

Rabbit Serotonin/5 Hydroxytryptamine ELISA kit is suitable for detecting samples from rabbit species. Serotonin/5 Hydroxytryptamine can also be called 5HT, Serotonin, N-Methyl-Gamma, 5 HT, 5-HT.

Products

Specifications of Rabbit Serotonin/5 Hydroxytryptamine ELISA kit

Product Information

Cat. No.

E04H0106

Product Name

Rabbit Serotonin/5 Hydroxytryptamine ELISA kit

Species

Rabbit

Product Size

48 Tests / 96 Tests

Concentration

25-500ng/mL

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

25 ng/mL

1 vial

STANDARD C (0.5mL)

50 ng/mL

1 vial

STANDARD D (0.5mL)

100 ng/mL

1 vial

STANDARD E (0.5mL)

250 ng/mL

1 vial

STANDARD F (0.5mL)

500 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

5 HT ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- 5 HT antibody and an 5 HT-HRP conjugate. The assay sample and buffer are incubated together with 5 HT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the 5 HT concentration since 5 HT from samples and 5 HT-HRP conjugate compete for the anti-5 HT antibody binding site. Since the number of sites is limited, as more sites are occupied by 5 HT from the sample, fewer sites are left to bind 5 HT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 5 HT concentration in each sample is interpolated from this standard curve.


Quality Control on Rabbit Serotonin/5 Hydroxytryptamine ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

89-114.79%

Linearity

Diluent Ratio

Range %

1:2

96-116

1:4

91-109

1:8

88-103

Specificity/Cross-reactivity

No significant cross-reactivity or interference between 5 HT and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rabbit Serotonin/5 Hydroxytryptamine ELISA kit

Summary of the Assay Procedure for Rabbit Serotonin/5 Hydroxytryptamine ELISA kit

Citations of Rabbit Serotonin/5 Hydroxytryptamine ELISA kit

E04H0106 has been referenced in the below publications:

经皮穴位电刺激对正畸家兔外周和中枢痛觉调控物质 5-羟色胺与P 物质的影响.

经皮穴位电剌激干预家兔正畸牙痛的作用及对脑脊液0-EP、5-HT影响的研究.

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