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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rabbit Hypoxia Inducible Factor 1, Alpha ELISA kit (E04H0061)

E04H0061 Rabbit Hypoxia Inducible Factor 1, Alpha ELISA kit

Rabbit Hypoxia Inducible Factor 1, Alpha ELISA kit is suitable for detecting samples from rabbit species. Hypoxia Inducible Factor 1, Alpha can also be called HIF1A, HIF-1-alpha, HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78, hypoxia inducible factor 1 alpha subunit, hypoxia inducible factor 1 subunit alpha, HIF-1α, HIF 1α, HIF1α.

Products

Specifications of Rabbit Hypoxia Inducible Factor 1, Alpha ELISA kit

Product Information

Cat. No.

E04H0061

Product Name

Rabbit Hypoxia Inducible Factor 1, Alpha ELISA kit

Species

Rabbit

Product Size

48 Tests / 96 Tests

Concentration

1.0-25ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

1.0 ng/mL

1 vial

STANDARD C (0.5mL)

2.5 ng/mL

1 vial

STANDARD D (0.5mL)

5.0 ng/mL

1 vial

STANDARD E (0.5mL)

10 ng/mL

1 vial

STANDARD F (0.5mL)

25 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

HIF 1Alpha ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- HIF 1Alpha antibody and an HIF 1Alpha-HRP conjugate. The assay sample and buffer are incubated together with HIF 1Alpha-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HIF 1Alpha concentration since HIF 1Alpha from samples and HIF 1Alpha-HRP conjugate compete for the anti-HIF 1Alpha antibody binding site. Since the number of sites is limited, as more sites are occupied by HIF 1Alpha from the sample, fewer sites are left to bind HIF 1Alpha-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HIF 1Alpha concentration in each sample is interpolated from this standard curve.


Quality Control on Rabbit Hypoxia Inducible Factor 1, Alpha ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

89-114.79%

Linearity

Diluent Ratio

Range %

1:2

96-116

1:4

91-109

1:8

88-103

Specificity/Cross-reactivity

No significant cross-reactivity or interference between HIF 1Alpha and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rabbit Hypoxia Inducible Factor 1, Alpha ELISA kit

Summary of the Assay Procedure for Rabbit Hypoxia Inducible Factor 1, Alpha ELISA kit

Citations of Rabbit Hypoxia Inducible Factor 1, Alpha ELISA kit

E04H0061 has been referenced in the below publications:

Influence of exogenous putrescine and cadaverine on pro-inflammatory factors in the peripheral blood of rabbits.

Effects of Tongxinluo on hypoxia tolerance of rabbit.

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