E04A0206 Rabbit Angiotensin (1-7) ELISA kit
Rabbit Angiotensin (1-7) ELISA kit is suitable for detecting samples from rabbit species. Angiotensin (1-7) can also be called Ang 1-7, Angiotensin 1-7, ANG (1-7).
Rabbit Angiotensin (1-7) ELISA kit
48 Tests / 96 Tests
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
STANDARD A (0.5mL)
STANDARD B (0.5mL)
STANDARD C (0.5mL)
STANDARD D (0.5mL)
STANDARD E (0.5mL)
STANDARD F (0.5mL)
WASH SOLUTION (100 x)
Principle of the Assay
ANG (1-7) ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- ANG (1-7) antibody and an ANG (1-7)-HRP conjugate. The assay sample and buffer are incubated together with ANG (1-7)-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ANG (1-7) concentration since ANG (1-7) from samples and ANG (1-7)-HRP conjugate compete for the anti-ANG (1-7) antibody binding site. Since the number of sites is limited, as more sites are occupied by ANG (1-7) from the sample, fewer sites are left to bind ANG (1-7)-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ANG (1-7) concentration in each sample is interpolated from this standard curve.
Coefficient of Variance
Intra Variation% ＜10%
Inter Variation% ＜12%
No significant cross-reactivity or interference between ANG (1-7) and analogues was observed.
E04A0206 has been referenced in the below publications:
Effects of Different Doses of Perindopril on Cardiac Function and ACE2/Ang-(1-9)/Ang-(1-7) Axis of Ischemic Cardiac Dysfunction Rabbits.
Effect of perindopril on stress-induced cardiomyopathy in animal models.
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