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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rabbit Angiotensin (1-7) ELISA kit (E04A0206)

E04A0206 Rabbit Angiotensin (1-7) ELISA kit

Rabbit Angiotensin (1-7) ELISA kit is suitable for detecting samples from rabbit species. Angiotensin (1-7) can also be called Ang 1-7, Angiotensin 1-7, ANG (1-7).

Products

Specifications of Rabbit Angiotensin (1-7) ELISA kit

Product Information

Cat. No.

E04A0206

Product Name

Rabbit Angiotensin (1-7) ELISA kit

Species

Rabbit

Product Size

48 Tests / 96 Tests

Concentration

50-1000ng/mL

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

50 ng/mL

1 vial

STANDARD C (0.5mL)

100 ng/mL

1 vial

STANDARD D (0.5mL)

250 ng/mL

1 vial

STANDARD E (0.5mL)

500 ng/mL

1 vial

STANDARD F (0.5mL)

1000 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

ANG (1-7) ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- ANG (1-7) antibody and an ANG (1-7)-HRP conjugate. The assay sample and buffer are incubated together with ANG (1-7)-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ANG (1-7) concentration since ANG (1-7) from samples and ANG (1-7)-HRP conjugate compete for the anti-ANG (1-7) antibody binding site. Since the number of sites is limited, as more sites are occupied by ANG (1-7) from the sample, fewer sites are left to bind ANG (1-7)-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ANG (1-7) concentration in each sample is interpolated from this standard curve.


Quality Control on Rabbit Angiotensin (1-7) ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

89-114.79%

Linearity

Diluent Ratio

Range %

1:2

96-116

1:4

91-109

1:8

88-103

Specificity/Cross-reactivity

No significant cross-reactivity or interference between ANG (1-7) and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rabbit Angiotensin (1-7) ELISA kit

Summary of the Assay Procedure for Rabbit Angiotensin (1-7) ELISA kit

Citations of Rabbit Angiotensin (1-7) ELISA kit

E04A0206 has been referenced in the below publications:

Effects of Different Doses of Perindopril on Cardiac Function and ACE2/Ang-(1-9)/Ang-(1-7) Axis of Ischemic Cardiac Dysfunction Rabbits.

Effect of perindopril on stress-induced cardiomyopathy in animal models.

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