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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rabbit 17 Hydroxycorticosteroids ELISA kit (E04A0659)

E04A0659 Rabbit 17 Hydroxycorticosteroids ELISA kit

Rabbit 17 Hydroxycorticosteroids ELISA kit is suitable for detecting samples from rabbit species. 17 Hydroxycorticosteroids can also be called 17 OHCSs, 17 Hydroxycorticosteroids, 17 OHCS.

Products

Rabbit 17 Hydroxycorticosteroids ELISA kit

Product Information

Cat. No.

E04A0659

Product Name

Rabbit 17 Hydroxycorticosteroids ELISA kit

Species

Rabbit

Product Size

48 Tests / 96 Tests

Concentration

0.5-10nmol/mL

Sensitivity

0.1 nmol/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 nmol/mL

1 vial

STANDARD B (0.5mL)

0.5 nmol/mL

1 vial

STANDARD C (0.5mL)

1.0 nmol/mL

1 vial

STANDARD D (0.5mL)

2.5 nmol/mL

1 vial

STANDARD E (0.5mL)

5.0 nmol/mL

1 vial

STANDARD F (0.5mL)

10 nmol/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

17 OHCS ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- 17 OHCS antibody and an 17 OHCS-HRP conjugate. The assay sample and buffer are incubated together with 17 OHCS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the 17 OHCS concentration since 17 OHCS from samples and 17 OHCS-HRP conjugate compete for the anti-17 OHCS antibody binding site. Since the number of sites is limited, as more sites are occupied by 17 OHCS from the sample, fewer sites are left to bind 17 OHCS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 17 OHCS concentration in each sample is interpolated from this standard curve.


Quality Control on Rabbit 17 Hydroxycorticosteroids ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

89-114.79%

Linearity

Diluent Ratio

Range %

1:2

96-116

1:4

91-109

1:8

88-103

Specificity/Cross-reactivity

No significant cross-reactivity or interference between 17 OHCS and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rabbit 17 Hydroxycorticosteroids ELISA kit

Summary of the Assay Procedure for Rabbit 17 Hydroxycorticosteroids ELISA kit

Citations of Rabbit 17 Hydroxycorticosteroids ELISA kit

E04A0659 has been referenced in the below publications:

脾肾阳虚型IBS-D大鼠模型探索及温肾健脾方干预CRF信号通路的机制研究.

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