Rabbit 17 Hydroxycorticosteroids ELISA kit

BlueGene Biotech Rabbit 17 Hydroxycorticosteroids ELISA kit

E04A0659 Rabbit 17 Hydroxycorticosteroids ELISA kit

Rabbit 17 Hydroxycorticosteroids ELISA kit is suitable for detecting samples from rabbit species. 17 Hydroxycorticosteroids can also be called 17 OHCSs, 17 Hydroxycorticosteroids, 17 OHCS.

Products

Product Information
Cat. No.	E04A0659
Product Name	Rabbit 17 Hydroxycorticosteroids ELISA kit
Species	Rabbit
Product Size	48 Tests / 96 Tests
Concentration	0.5-10nmol/mL
Sensitivity	0.1 nmol/mL
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 nmol/mL	1 vial
STANDARD B (0.5mL)	0.5 nmol/mL	1 vial
STANDARD C (0.5mL)	1.0 nmol/mL	1 vial
STANDARD D (0.5mL)	2.5 nmol/mL	1 vial
STANDARD E (0.5mL)	5.0 nmol/mL	1 vial
STANDARD F (0.5mL)	10 nmol/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

17 OHCS ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- 17 OHCS antibody and an 17 OHCS-HRP conjugate. The assay sample and buffer are incubated together with 17 OHCS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the 17 OHCS concentration since 17 OHCS from samples and 17 OHCS-HRP conjugate compete for the anti-17 OHCS antibody binding site. Since the number of sites is limited, as more sites are occupied by 17 OHCS from the sample, fewer sites are left to bind 17 OHCS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 17 OHCS concentration in each sample is interpolated from this standard curve.

Quality Control on Rabbit 17 Hydroxycorticosteroids ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	89-114.79%
Linearity	Diluent Ratio	Range %
	1:2	96-116
	1:4	91-109
	1:8	88-103
Specificity/Cross-reactivity	No significant cross-reactivity or interference between 17 OHCS and analogues was observed.