E04G0301 Rabbit Glycosaminoglycans ELISA kit
Rabbit Glycosaminoglycans ELISA kit is suitable for detecting samples from rabbit species. Glycosaminoglycans can also be called GAGs, mucopolysaccharides.
Specifications of Rabbit Glycosaminoglycans ELISA kit
Product Information | |
Cat. No. | E04G0301 |
Product Name | Rabbit Glycosaminoglycans ELISA kit |
Species | Rabbit |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100ng/mL |
Sensitivity | 1.0 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 10 ng/mL | 1 vial |
STANDARD D (0.5mL) | 25 ng/mL | 1 vial |
STANDARD E (0.5mL) | 50 ng/mL | 1 vial |
STANDARD F (0.5mL) | 100 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
GAGs ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- GAGs antibody and an GAGs-HRP conjugate. The assay sample and buffer are incubated together with GAGs-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GAGs concentration since GAGs from samples and GAGs-HRP conjugate compete for the anti-GAGs antibody binding site. Since the number of sites is limited, as more sites are occupied by GAGs from the sample, fewer sites are left to bind GAGs-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GAGs concentration in each sample is interpolated from this standard curve. |
Quality Control on Rabbit Glycosaminoglycans ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 89-114.79% | |
Linearity | Diluent Ratio | Range % |
1:2 | 96-116 | |
1:4 | 91-109 | |
1:8 | 88-103 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between GAGs and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rabbit Glycosaminoglycans ELISA kit
Citations of Rabbit Glycosaminoglycans ELISA kit
E04G0301 has been referenced in the below publications:
Low-Molecular-Weight Heparin-Functionalized Chitosan-Chondroitin Sulfate Hydrogels for Controlled Release of TGF-β3 and in vitro Neocartilage Formation.
Integrated Trilayered Silk Fibroin Scaffold for Osteochondral Differentiation of Adipose-Derived Stem Cells.
