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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rabbit Platelet activating factor ELISA kit

E04P0589 Rabbit Platelet activating factor ELISA kit

Rabbit Platelet activating factor ELISA kit is suitable for detecting samples from rabbit species. Platelet activating factor can also be called PAF-Acether, AGEPC, Acetyl-Glyceryl-Ether-Phosphorylcholine, PAF.

Specifications of Rabbit Platelet activating factor ELISA kit

Product Information

Cat. No.

E04P0589

Product Name

Rabbit Platelet activating factor ELISA kit

Species

Rabbit

Product Size

48 Tests / 96 Tests

Concentration

1.0-25ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

1.0 ng/mL

1 vial

STANDARD C (0.5mL)

2.5 ng/mL

1 vial

STANDARD D (0.5mL)

5.0 ng/mL

1 vial

STANDARD E (0.5mL)

10 ng/mL

1 vial

STANDARD F (0.5mL)

25 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

PAF ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- PAF antibody and an PAF-HRP conjugate. The assay sample and buffer are incubated together with PAF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PAF concentration since PAF from samples and PAF-HRP conjugate compete for the anti-PAF antibody binding site. Since the number of sites is limited, as more sites are occupied by PAF from the sample, fewer sites are left to bind PAF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PAF concentration in each sample is interpolated from this standard curve.


Quality Control on Rabbit Platelet activating factor ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

89-114.79%

Linearity

Diluent Ratio

Range %

1:2

96-116

1:4

91-109

1:8

88-103

Specificity/Cross-reactivity

No significant cross-reactivity or interference between PAF and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rabbit Platelet activating factor ELISA kit

Summary of the Assay Procedure for Rabbit Platelet activating factor ELISA kit

Citations of Rabbit Platelet activating factor ELISA kit

E04P0589 has been referenced in the below publications:

The Study about Mechanism of Onion stalk extraction on Prevention and Treatment of Rabbits’Traumatic Deep Vein Thrombosis.

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